A device and method for offline collection of human exhaled gas

Abstract

The invention discloses an off-line collection device and method for human exhaled gas, which can be used to identify disease-related volatile organic compounds (VOCs) components in exhaled gas by cooperating with gas chromatography tandem mass spectrometry, proton transfer reaction mass spectrometry and other instruments. The device includes a gas inhalation pipeline, an exhaled gas collection pipeline and a dilution pipeline. The subject inhales the air purified by the gas inhalation pipeline and stored in the buffer air bag, and then exhales into the exhaled gas collection pipeline, and the buffer gas is sampled. There are two methods of collection, bag and adsorption tube. The exhaled gas off-line collection device of the present invention can realize the standardized on-site rapid collection of wide-spectrum VOCs in human exhaled gas in batches within a large group of people, and cooperate with multi-type mass spectrometry equipment to realize early disease screening, continuous monitoring of disease degree, and drug treatment effect test Wait.

Description

technical field [0001] The invention relates to an off-line collection device for human exhaled gas, in particular to an instrument that can cooperate with gas chromatography tandem mass spectrometry (GC-MS), proton transfer reaction mass spectrometry (PTR-MS) and other instruments to be used for disease-related volatile organic compounds ( A device for identifying components of VOCs) belongs to the field of sample collection in hygiene. Background technique [0002] The research and application of the analysis of VOCs components in human exhaled breath in the diagnosis of chronic diseases is attracting more and more attention from the society. For example, acetone, dimethyl sulfide, isoprene, butanol, methyl nitrate, and some long-chain alkanes and benzene series were found to have significantly abnormal levels in the exhaled breath of diabetic patients; benzene, propanol, Styrene, pentane, etc. have been identified as potential markers of lung cancer in many studies; alde...

AI Technical Summary

Problems solved by technology

[0003] The use of exhaled breath collection devices for VOCs analysis and disease diagnosis still has the following problems: (1) Although a large number of studies have been carried out, the types and levels of exhaled breath VOCs related to the same disease screened by different studies have different Big difference (Buszewski, B., et al., Biomed Chromatogr, 2007.21(6): p.553-66.)

One of the key reasons for this phenomenon is the lack of a unified exhaled gas collection device and operating process, which seriously affects the comparability of exhaled gas VOCs analysis, and also makes it difficult to collect and analyze exhaled gas VOCs in batches.

(2) Due to the difference in principle, the range of charge-to-mass ratio of VOCs analyzed by different equipment is different

(3) At present, there are three main off-line exhaled gas collection systems (Van Berkel, J.J., et al., Respir Med, 2010.104(4): p.557-63; Herbig, J., et al., J Breath Res ,2008.2(3):p.037008; Herbig,J.,et al.,J Breath Res,2009.3(2):p.027004.): One is to use the Tedlar air bag to directly collect the exhaled breath of the subject, This method has a simple device and is easy to operate, but it cannot rule out the influence of external VOCs inhalation on low-concentration exhaled VOCs, cannot avoid the impact of high humidity in exhaled air on the accuracy of analytical equipment, and cannot be used for long-distance transportation and storage of samples; The second is to use the face mask method to collect the exhaled breath of the subject, but this method is costly, time-consuming and complicated to operate, and is not suitable for disease diagnosis and exhaled VOCs research of large groups of people; the third is to use solid adsorption materials Combined with thermal desorption method for collection and analysis, but this method is seriously insufficient for the analysis of VOCs with low charge-to-mass ratio

(4) During the collection process, the subject may feel uncomfortable due to the pressure of the sampling line, and cannot perform stretched active breathing, which affects the consistency of sample collection conditions and the compliance of the subject

This method still has the following problems: First, in this method, the subjects need to hold their breath for 5 seconds and exhale forcefully, which reduces the adaptability and repeatability of the crowd, and the obtained exhaled VOCs may have large differences; Secondly, this method first uses the air bag to collect, then transfers to the adsorption tube, and performs mass spectrometry detection through thermal desorption treatment, which increases the sampling time and affects the accuracy of the detection results, and is not suitable for sampling in multiple regions and large groups of people; Third, this method is only matched with GC-MS to detect exhaled VOCs, and its ability to detect VOCs with low nucleoplasmic ratio is very limited

However, this device does not consider the impact of high humidity and VOCs in the environment on subsequent analysis

In addition, the devices used in this patent can only be used in conjunction with a single type of VOCs analysis instrument, and the types of VOCs detected are limited by the instrument, which seriously affects the screening of key VOCs components

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