A method for preparing hemp cellulose enzymatic hydrolyzate coupled protein for cultural relics detection
A technology of hemp cellulose and enzymatic hydrolyzate, which is applied in the field of preparation of hemp cellulose enzymatic hydrolyzate coupled to protein, can solve problems such as animal immunity, achieve the effects of improving purity, saving production costs, and speeding up the speed of enzymatic hydrolysis
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Embodiment 1
[0028] 1) Take 10 g of hemp fiber, grind it for 3 minutes, put it in a beaker, add 45 mL of benzene and 20 mL of ethanol, and soak for 6 h to degrease the fiber. After the treatment, the mixed solution is evaporated under reduced pressure to recover benzene and Ethanol, the degreased hemp fiber was washed 5 times with deionized water, and dried in an oven at 50 °C.
[0029] 2) Take 5 g of dried hemp fiber powder, add 100 mL of acetone and 50 mL of deionized water, stir and mix evenly, then add 10 mL of formic acid dropwise, soak for 2 h, and centrifuge the solution at 1000 r / min. Take the bottom precipitate and continue to treat it with the above solution and centrifuge, repeat the operation 4 times to obtain the final precipitate, wash it with deionized water 5 times, add 200 mL of 7% sodium hydroxide, heat it in a water bath at 75 °C for 12 h, and then filter it with suction , the precipitate was washed 5 times with deionized water.
[0030] 3.) Take the final precipitate o...
Embodiment 2
[0037] 1) Take 10 g of hemp fiber, grind it for 4 minutes, put it in a beaker, add 50 mL of benzene and 25 mL of ethanol, soak for 8 h to degrease the fiber, and evaporate the mixed solution under reduced pressure to recover benzene and Ethanol, the degreased hemp fiber was washed 5 times with deionized water, and dried in an oven at 50 °C.
[0038] 2) Take 5 g of dried hemp fiber powder, add 100 mL of acetone and 50 mL of deionized water, stir and mix evenly, then add 10 mL of formic acid dropwise, soak for 2 h, and centrifuge the solution at 1000 r / min. Take the bottom precipitate and continue to treat it with the above solution and centrifuge, repeat the operation 4 times to obtain the final precipitate, wash it with deionized water 5 times, add 200 mL of 8% sodium hydroxide, heat it in a water bath at 75 °C for 12 h, and then filter it with suction , the precipitate was washed 5 times with deionized water.
[0039] 3) Take the final precipitate obtained in step 2, add 10%...
Embodiment 3
[0046] 1) Take 10 g of hemp fiber, grind it for 5 minutes, put it in a beaker, add 55 mL of benzene and 30 mL of ethanol, and soak for 10 h to degrease the fiber. After the treatment, the mixed solution is evaporated under reduced pressure to recover benzene and Ethanol, the degreased hemp fiber was washed 5 times with deionized water, and dried in an oven at 50 °C.
[0047] 2) Take 5 g of dried hemp fiber powder, add 100 mL of acetone and 50 mL of deionized water, stir and mix evenly, then add 10 mL of formic acid dropwise, soak for 2 h, and centrifuge the solution at 1000 r / min. Take the bottom precipitate and continue to treat it with the above solution and centrifuge, repeat the operation 4 times to obtain the final precipitate, wash it with deionized water 5 times, add 200 mL of 9% sodium hydroxide, heat it in a water bath at 75 °C for 12 h, and then filter it with suction , the precipitate was washed 5 times with deionized water.
[0048] 3) Take the final precipitate o...
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