Method for extracting and purifying astragaloside IV in Astragalus root

A technology for astragaloside IV and astragalus root, which is applied in the field of biological extraction, can solve the problems of low purity and low yield of astragaloside IV, and achieve the effects of high purity, short processing time and high yield

Inactive Publication Date: 2017-11-21
安徽龙津生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the extraction methods of astragaloside IV mainly include water extraction, alcohol extraction, heating extraction, ultrasonic extraction, etc. The product astragaloside IV extracted by the prior art methods generally has the disadvantages of low yield and low purity, and cannot be used for high Application in value-added products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for extracting and purifying astragaloside IV from Astragalus root, comprising the following steps:

[0029] (1) Pulverization: Pulverize the roots of Astragalus membranaceus through a 10-mesh sieve to obtain coarse powder of Astragalus membranaceus roots;

[0030] (2) Pretreatment: Microwave the Astragalus root powder for 30 minutes at a temperature of 30°C and a vacuum of -0.05MPa;

[0031] (3) Extraction and concentration: add the pretreated astragalus root powder into water according to the ratio of material to liquid 1:12, stir well and evenly, and firstly treat it with ultra-high pressure impingement flow under the condition of loading pressure of 250MPa and impact distance of 4mm for 5 Once again, under the condition that the electric field strength is 15kV / cm, and the number of pulses is 10, the high-voltage pulse electric field is treated for 40s, and the extraction is refluxed for 1h, and the extract is filtered out. The frequency is 30kHz / 15kHz, the...

Embodiment 2

[0040] A method for extracting and purifying astragaloside IV from Astragalus root, comprising the following steps:

[0041] (1) Pulverization: pulverize the root of Astragalus membranaceus through a 15-mesh sieve to obtain coarse powder of Astragalus membranaceus root;

[0042] (2) Pretreatment: Microwave the astragalus root powder for 25 minutes at a temperature of 35°C and a vacuum of -0.06MPa;

[0043] (3) Extraction and concentration: add the pretreated astragalus root powder into water according to the ratio of material to liquid 1:14, stir well and evenly, and firstly treat it with ultra-high pressure impingement flow under the conditions of loading pressure of 300MPa and impact distance of 3mm4 Once again, under the condition that the electric field strength is 18kV / cm, and the number of pulses is 12, the high-voltage pulse electric field is treated for 30s, refluxed for 1.5h, and the extract is filtered out. The alternating frequency is 35kHz / 20kHz, the alternating w...

Embodiment 3

[0052] A method for extracting and purifying astragaloside IV from Astragalus root, comprising the following steps:

[0053] (1) Pulverization: Pulverize the roots of Astragalus membranaceus through a 20-mesh sieve to obtain coarse powder of Astragalus membranaceus roots;

[0054] (2) Pretreatment: microwave the astragalus root powder for 20 minutes at a temperature of 40°C and a vacuum of -0.08MPa;

[0055] (3) Extraction and concentration: add the pretreated astragalus root powder into water according to the ratio of solid to liquid 1:16, stir well and evenly, and firstly treat it with ultra-high pressure impingement flow under the condition of loading pressure of 350MPa and impact distance of 2mm. Once again, under the condition that the electric field strength is 20kV / cm and the number of pulses is 15, the high-voltage pulse electric field is treated for 20s, refluxed for 2h, and the extract is filtered out. The frequency is 40kHz / 25kHz, the dual-frequency ultrasonic alte...

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PUM

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Abstract

The invention discloses a method for extracting and purifying astragaloside IV in Astragalus root. The method comprises the steps of crushing, pretreatment, extraction, concentrating, adsorption separation, decolorizing, concentrating crystallization, centrifugation, recrystallization, centrifugation and drying. The method has the advantages of easily available raw material, extraction from the Astragalus root having rich sources, high yield of above 0.1% and high purity of above 99% of the obtained product astragaloside IV, high extraction efficiency, short treatment time, and suitableness for large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of biological extraction, and in particular relates to a method for extracting and purifying astragaloside IV from astragalus root. Background technique [0002] Astragaloside IV, Chinese alias: Astragaloside IV, English name: Astragaloside IV. [0003] Product properties: white needle crystal, molecular formula: C41H68O14, CAS number: 84687-43-4 molecular weight: 784.9702, relative density: 1.39g / cm 3 , Melting point: 295-296°C. [0004] Astragaloside IV sources Leguminous astragalus astragalus alexandrinus boiss root, astragalus astragalus dissectus root, astragalus membranaceus (fisch.) bungede root, astragalus sieversianus pall. root, astragalus spinosus vahl aboveground part. [0005] Pharmacological effects: Astragaloside IV enhances immunity, increases energy, resists fatigue, causes mutations, protects the liver, and inhibits osteoclasts. [0006] At present, the extraction methods of astragaloside IV main...

Claims

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Application Information

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IPC IPC(8): C07J53/00
CPCC07J53/004
Inventor 钟玉林
Owner 安徽龙津生物科技有限公司
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