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Haemophilus paragallinarum and application thereof

A technology of Haemophilus gallinarum and microbial strains is applied in the direction of bacteria, antibacterial drugs, veterinary vaccines, etc., which can solve the problems of increased elimination rate, decreased egg production of laying hens, and decreased quality of broilers, and achieves stable biological Characteristics, vaccine safety and reliability, and the effect of good immunogenicity

Inactive Publication Date: 2017-11-17
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Haemophilus paragallinarum can cause a decrease in the egg production of laying hens, a decrease in the quality of broiler chickens, stunted growth of growing chickens, and an increase in the elimination rate, causing large economic losses to the chicken industry and seriously affecting the development of the chicken industry

Method used

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  • Haemophilus paragallinarum and application thereof
  • Haemophilus paragallinarum and application thereof
  • Haemophilus paragallinarum and application thereof

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Experimental program
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Effect test

Embodiment 1

[0020] Embodiment 1, the isolation and identification of Haemophilus paragallinarum

[0021] 1.1 Disease data collection

[0022] The disease material came from a laying hen that had dyspnea and swelling of the head and face in a large laying hen farm in Weihui City, Henan Province in April 2017.

[0023] 1.2 Preparation of medium

[0024] TSB liquid medium: Dissolve 30g of TSB broth powder (Tryptic Soy broth, tryptone soybean broth) in 1000mL of ultrapure water, sterilize at 115°C for 15min, add 50mL of fetal bovine serum, sterile mass fraction of 1% NAD (Nicotinamide adenine dinucleotide, nicotinamide adenine dinucleotide, coenzyme Ⅰ) 1mL;

[0025] TSA solid medium: Dissolve 40 g of TSA agar powder (Tryptic Soy Agar, tryptone soybean agar) in 1000 mL of ultrapure water, sterilize at 115 °C for 15 min, add 50 mL of fetal bovine serum, 1 mL of sterile mass fraction 1% NAD ( Add as needed), and store at 4°C for later use.

[0026] 1.3 Isolation and culture of bacteria

[0...

Embodiment 2

[0045] Embodiment 2, the preparation of vaccine, safety and efficacy test

[0046] 2.1 Preparation of vaccine

[0047] Inoculate Haemophilus paragallinarum strain HNHpg1 into TSB liquid medium, culture in a shaker at 37°C, collect the culture after 24 hours, measure the cell concentration, and adjust the number of Haemophilus paragallinarum in the culture to 2× 10 9 CFU / mL, add 2mL formaldehyde solution (mass fraction: 37%) for every 1000mL culture, inactivate at 37°C for 12h to prepare the vaccine stock solution; then add aluminum hydroxide adjuvant (Sigma, F5881) equal to the volume of the vaccine stock solution , mixed to make Haemophilus paragallinarum inactivated vaccine, the number of Haemophilus paragallinarum bacteria in the vaccine is about 1×10 9 CFU / mL.

[0048] 2.2 Vaccine safety test:

[0049] Twenty 42-day-old healthy chickens were selected and divided into 4 groups with 5 chickens in each group. Vaccine group 3 groups, each group was subcutaneously injected...

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Abstract

The invention discloses a haemophilus paragallinarum and application thereof. The haemophilus paragallinarum is haemophilus paragallinarum HNHpg1, the preservation number is CGMCC NO:13859, the preservation date is May 26th, 2017, the preservation place is China General Microbiological Culture Collection Center and the preservation address is Beijing, China. The haemophilus paragallinarum HNHpg1 is separated from secreta of sinus infraorbitalis of a laying hen suffering from typical respiratory symptoms and head and facial swelling, has stable biological characteristics, has stronger pathogenicity on chicks, can result in morbidity and death of the chicks and has good immunogenicity. A vaccine prepared by utilizing the haemophilus paragallinarum HNHpg1 disclosed by the invention is safe and reliable and has a better protective effect on chicken infectious rhinitis.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a strain of Haemophilus paragallinarum and its application. Background technique [0002] Haemophilus paragallinarum (Haemophilus paragallinarum, HPg) is a short Gram-negative bacillus of Pasteurellaceae Avibacterium (Avibacterium), the pathogen of chicken infectious coryza (Infectious coryza, Ic), causing chicken acute Or subacute respiratory infection, the clinical manifestations are nasal cavity, infraorbital sinus and upper trachea inflammation, tearing, nasal discharge, and dyspnea. Sick chickens sneeze, shake their heads, and have edema on one or both sides of the face. The disease is widely distributed all over the world. Beach first reported the disease in 1920, and then De Bliekc isolated Hpg for the first time. The pathogen was first isolated in Beijing in 1987 in my country, and reports of this bacteria were successively isolated in Hebei, Liaoning, Shandon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/102A61P31/04C12R1/21
CPCA61K39/102A61K2039/521A61K2039/552C12N1/205C12R2001/21
Inventor 张青娴徐引弟王治方郎利敏张立宪李海利朱文豪焦文强王克领游一
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
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