Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of magnetic mesoporous molecular sieve based nNOS-PSD-95 uncoupler surface molecularly imprinted polymer

A technology of mesoporous molecular sieve and uncoupling agent, which is applied in the directions of alkali metal compounds, chemical instruments and methods, alkali metal oxides/hydroxides, etc. Complex, many interfering components, and good reproducibility

Inactive Publication Date: 2017-11-17
NANJING MEDICAL UNIV
View PDF5 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Technical problem to be solved: Aiming at the inefficiency and cumbersomeness of traditional Chinese medicine activity screening and the shortcomings of traditional molecularly imprinted polymers, the present invention provides a magnetic mesoporous molecular sieve based nNOS-PSD-95 uncoupling agent surface imprinted polymer The preparation method aims to find anti-stroke candidate drugs with significant activity and less toxic and side effects, and at the same time provide new ideas and methods for the specificity and high-efficiency screening of active ingredients of traditional Chinese medicine

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of magnetic mesoporous molecular sieve based nNOS-PSD-95 uncoupler surface molecularly imprinted polymer
  • Preparation method of magnetic mesoporous molecular sieve based nNOS-PSD-95 uncoupler surface molecularly imprinted polymer
  • Preparation method of magnetic mesoporous molecular sieve based nNOS-PSD-95 uncoupler surface molecularly imprinted polymer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1). Weigh 2.7g FeCl 3 ·6H 2 O and 7.2g NaAc were dissolved in 100mL ethylene glycol with magnetic stirring until a uniform yellow solution was obtained, transferred to a tetrafluoroethylene autoclave, left standing at 200°C for 8h, cooled at room temperature, washed 6 times with absolute ethanol, magnetically separated, and vacuum-dried at 45°C to constant weight, get Fe 3 o4 powder.

[0038] (2). Weigh the obtained Fe 3 o 4 Ultrasonically disperse 0.1g powder in 80mL ethanol, add 20mL deionized water and 1mL concentrated ammonia water, add dropwise 0.1gTEOS to the solution under magnetic stirring, stir at room temperature for 6h, wash with ethanol and deionized water, and redisperse in 60mL ethanol and 80mL deionized water , 1g concentrated ammonia water mixed solution, add 0.3gCTAB, stir at room temperature for 30min, then dropwise add 0.4gTEOS, continue to stir for 6h, the rotation speed is 500rpm, the TEOS addition rate is one drop every 2s, and finally collect...

Embodiment 2

[0044] (1). Weigh 2.7g FeCl 3 ·6H 2 O and 7.2g NaAc were dissolved in 100mL ethylene glycol with magnetic stirring until a uniform yellow solution was obtained, transferred to a tetrafluoroethylene autoclave, left standing at 200°C for 8h, cooled at room temperature, washed 6 times with absolute ethanol, magnetically separated, and vacuum-dried at 45°C to constant weight, get Fe 3 o 4 powder.

[0045] (2). Weigh the obtained Fe 3 o 4 Ultrasonically disperse 0.1g powder in 80mL ethanol, add 20mL deionized water and 1mL concentrated ammonia water, add dropwise 0.1gTEOS to the solution under magnetic stirring, stir at room temperature for 6h, wash with ethanol and deionized water, and redisperse in 60mL ethanol and 80mL deionized water , 1g concentrated ammonia solution, add 0.3gCTAB, stir at room temperature for 30min, then dropwise add 0.4gTEOS, continue to stir for 6h, the rotation speed is 600rpm, the TEOS addition rate is one drop every 10s, and then collect the product...

Embodiment 3

[0051] (1). Weigh 2.7g FeCl 3 ·6H 2 O and 7.2g NaAc were dissolved in 100mL ethylene glycol with magnetic stirring until a uniform yellow solution was obtained, transferred to a tetrafluoroethylene autoclave, left standing at 200°C for 8h, cooled at room temperature, washed 6 times with absolute ethanol, magnetically separated, and vacuum-dried at 45°C to constant weight, get Fe 3 o 4 powder.

[0052] (2). Weigh the obtained Fe 3 o 4 Ultrasonically disperse 0.1g powder in 80mL ethanol, add 20mL deionized water and 1mL concentrated ammonia water, add dropwise 0.1gTEOS to the solution under magnetic stirring, stir at room temperature for 6h, wash with ethanol and deionized water, and redisperse in 60mL ethanol and 80mL deionized water , 1g concentrated ammonia water mixed solution, add 0.3gCTAB, stir at room temperature for 30min, then dropwise add 0.4gTEOS, continue to stir for 6h, the rotation speed is 700rpm, TEOS drop rate is one drop every 15s, and finally collect the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of a magnetic mesoporous molecular sieve based nNOS-PSD-95 uncoupler surface molecularly imprinted polymer, and belongs to the technical field of surface molecularly imprinted polymer. The preparation method comprises the following steps: preparing a magnetic mesoporous molecular sieve with a core-shell structure; subjecting the prepared magnetic mesoporous silica microsphere to surface grafting modification; and finally preparing the nNOS-PSD-95 uncoupler surface molecularly imprinted polymer based on the magnetic mesoporous molecular sieve. The prepared magnetic mesoporous molecular sieve based nNOS-PSD-95 uncoupler surface molecularly imprinted polymer has the advantages that the mono-dispersing property is good, the specific surface area is large, the mesopores are regular and ordered; the adsorption and desorption dynamic characteristics are excellent, moreover, the polymer is stable, the repeatability is good, and selective capture and high efficient screening of nNOS-PSD-95 uncoupler in traditional Chinese medicine become possible.

Description

technical field [0001] The invention belongs to the technical field of molecularly imprinted polymers, in particular to a preparation method of a magnetic mesoporous molecular sieve surface imprinted polymer with specific recognition for ZL006. Background technique [0002] Cerebral infarction (ischemic stroke) has the characteristics of high mortality, high disability rate, high recurrence rate, etc., and seriously endangers human health. Under cerebral ischemic conditions, excessive release of excitatory amino acids (such as glutamate) causes excessive activation of N-methyl-D-aspartate receptors (NMDAR), pathologically through the NMDAR-PSD-95-nNOS pathway Nitric oxide (NO) is released, causing severe damage to cells. The study found that the nNOS-PSD-95 uncoupler 4-(2-hydroxy-3,5-dichlorobenzylamino)-2-hydroxybenzoic acid (ZL006) has a clear protective effect on cerebral ischemia (Nature Medicine 2010 , 16, 12: 1439-U123), with clear targets and few side effects, but b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08F285/00C08F220/14C08F220/56C08F226/06C08F222/38C08F292/00C08F230/08C08J9/26B01J20/26B01J20/28
CPCB01J20/103B01J20/268B01J20/28009B01J20/28019C08F285/00C08F292/00C08J9/26C08J2201/0424C08J2351/10C08F230/08C08F220/14C08F220/56C08F226/06C08F222/385
Inventor 陈立娜黄姣姣孙成红姚丹丹张宇张爱霞
Owner NANJING MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com