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Enrichment method of microbes used for biologically removing high-concentration nitrogen-containing compounds

A technology of nitrogen compounds and microorganisms, applied in the field of biological denitrification, can solve the problems of low concentration and inability to purify nitrogen-containing substances, and achieve the effects of improving stability, enriching microbial populations, and increasing activity

Inactive Publication Date: 2017-11-10
ZHEJIANG UNIV
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AI Technical Summary

Problems solved by technology

[0005] In the natural soil and water, there are several kinds of microorganisms with nitrification and denitrification properties, but because the concentration is too low, it cannot effectively purify the nitrogen-containing substances in the soil and water.

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  • Enrichment method of microbes used for biologically removing high-concentration nitrogen-containing compounds
  • Enrichment method of microbes used for biologically removing high-concentration nitrogen-containing compounds

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Embodiment Construction

[0033] The specific embodiments of the present invention will be described in detail below in conjunction with the technical solution and the drawings, but the present invention is not limited to the following examples.

[0034] The anaerobic sludge from pig slaughterhouse wastewater treatment plants, food processing wastewater treatment plants, landfill leachate treatment plants, and urban domestic sewage treatment plants all contain a certain concentration of various nitrifying / denitrifying microorganisms.

[0035] The preparation method of the inoculation source: mix the anaerobic sludge from the pig slaughterhouse wastewater treatment plant, food processing plant wastewater treatment plant, landfill leachate treatment plant, and municipal sewage treatment plant in a volume ratio of 1:1:1:1, After stirring, filtering, and centrifuging, the supernatant was taken, and the 1 / 10 volume of the liquid in the lower layer of the supernatant was used as the inoculation source.

[0036] The...

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Abstract

The invention relates to the field of biological removal of nitrogen, and aims to provide a enrichment method of microbes used for biologically removing high-concentration nitrogen-containing compounds. The method comprises the steps that a variety of practical anaerobic sludge from wastewater treatment plants is mixed, stirred, filtered and centrifuged, and then an inoculating source is obtained; chemical reagents are added into the inoculating source for inoculation in a microbial fuel cell, and then the microbial fuel cell runs; finally, the microbes used for biologically removing high-concentration nitrogen-containing compounds are enriched in the fuel cell. According to the enrichment method, the microbial fuel cell serves as a carrier, the nitrating and denitrifying microbes can stably grow and largely proliferated in a specific culture environment in a bioelectrochemistry mode, and are be absorbed on a cell electrode to form a dense membrane structure, and then the effect that the high-concentration nitrogen-containing compounds can be efficiently removed is achieved. In the method, a variety of practical wastewater is introduced, the microbial population is enriched, and the stability of the system is improved; the environment beneficial to the growth and propagation of the nitrating and denitrifying microbes is built, a selective effect is achieved on the microbial population, and the enrichment aim is achieved.

Description

Technical field [0001] The invention belongs to the field of biological denitrification, and relates to an enrichment method for biologically removing microorganisms containing high-concentration nitrogen compounds. Background technique [0002] Organic matter, nitrogen-containing substances, and phosphorus-containing substances are common water pollutants. Since my country implemented "One Control and Two Standards" in 2000, organic pollution in wastewater has been effectively curbed, and nitrogen pollution has become a major environmental problem facing my country. At present, the discharge of nitrogen pollutants in the country has far exceeded the environmental capacity of receiving water bodies, and nitrogen pollutants have also exceeded COD as the primary indicator affecting water environmental quality. Nitrogen pollution has endangered many industries such as agriculture, fishery, and tourism, and poses a serious threat to drinking water hygiene and food safety. The contr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/02C02F3/34
CPCC02F3/34C12N1/02
Inventor 成少安周永刚黄志鹏
Owner ZHEJIANG UNIV
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