Identification method for cucumber-sour cucumber alien addition line material

Abstract

The invention discloses an identification method for a cucumber-sour cucumber alien addition line material. The identification method comprises: carrying out backcross on a Cucumis allotriploid and diploid cultivation cucumber to obtain a cucumber-sour cucumber alien addition line candidate material, wherein a Cucumis amphidiploid new variety and diploid cucumber are subjected to reciprocal hybridization to obtain the Cucumis allotriploid; labeling a cucumber centromere specific repetitive sequence Type III by using a probe; taking the root tip of the cucumber-sour cucumber alien addition line candidate material, carrying out enzymolysis, and preparing a slice by using a flame drying method to obtain a metaphase chromosome slice having good dispersion; and denaturing the cucumber centromere specific repetitive sequence Type III probe, hybridizing the denatured probe onto the denatured metaphase chromosome slice, and making the Type III produce the clear and distinguishable fluorescent signal by using highly stringency hybridization conditions, such that the cucumber-sour cucumber alien addition line material and the number of the added sour cucumber chromosomes are identified. According to the present invention, the identification method creates the conditions for the transfer of the wild sour cucumber excellent gene in the alien addition line material and the cultivation of the new cucumber variety.

Description

1. Technical field [0001] The invention discloses an identification method for cucumber-sour cucumber isoadditional line materials, which is beneficial to the development of cucumber breeding research and belongs to the field of plant biotechnology. 2. Technical background [0002] Cucumber (Cucumis sativus L., 2n=2x=14) belongs to the Cucurbitaceae genus Cucumis and is an important vegetable crop worldwide. The genetic basis of cucumber is very narrow, and there is a lack of important resistance genes within the species. The existing cultivars are generally sensitive to biotic and abiotic stresses such as diseases, high and low temperature stress, etc. Improvement is especially important (Dijkhuizen et al., 1994; Staub et al., 2005). [0003] Cucumber is rich in wild resources, and the genus Melon belongs to contains more than 50 wild species (Sebastian et al., 2010). However, due to the differentiation of species (cucumber is the only species in this genus with chromosom...

AI Technical Summary

Problems solved by technology

[0008] The present invention uses the fluorescent in situ hybridization technique of the cucumber chromosome centromere-specific repeat sequence Type III to identify the additional pickled cucumber chromosomes and their numbers in the cucumber-acid cucumber heteroadditional line material, which is aimed at the tediousness of the previous identification methods for additional line materials, etc. Insufficient and lack of pickled cucumber chromosome-specific identification markers, invented a method to quickly identify additional pickled cucumber chromosomes and their number only through fluorescence in situ hybridization (FISH) of cucumber centromere-specific repeat sequence Type III

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