Betula microphylla var.paludosa embryonic callus induction method

An embryogenic callus and leaflet technology is applied in the field of plant tissue culture to achieve the effect of promoting breeding research

Inactive Publication Date: 2015-02-04
SHANGHAI SUNQIAO MODERN GREENHOUSE SEED & SEEDLING +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the technology of using the stem of Betula microphylla test-tube plantlets as explants to induce embryogenic cell generation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] A method for inducing embryogenic callus of Betula microphylla, the specific steps are as follows: (1) Select rooted seedlings of Betula microphylla tissue-cultured with a seedling age of 28 days and a height of 3 cm; (2) Cut the stem section of the rooted seedlings cultured into (3) Inoculate the explants in a medium containing mother liquor, 0.2mg / L 6-benzylaminoadenine, 0.5mg / L naphthaleneacetic acid and 500mg / L hydrolyzed casein Cultivate in protein dedifferentiation medium for 7 days to dedifferentiate and form callus. The culture temperature is 23°C, the light time is 12h / d, and the light intensity is 40umol / m 2 s; (4) Transfer the callus to the redifferentiation medium including the medium mother solution, 0.5mg / L 6-benzylaminoadenine and 0.02mg / L naphthaleneacetic acid for 10 days, and the culture temperature is 23℃ , the light time is 12h / d, and the light intensity is 40umol / m 2 ·s.

Embodiment 2

[0015] A method for inducing embryogenic callus of Betula microphylla, the specific steps are as follows: (1) select rooted seedlings of Betula microphylla tissue-cultured with a seedling age of 32 days and a seedling height of 5 cm; (2) cut the stem section of the rooted seedlings cultured (3) Inoculate the explants in a medium containing mother liquor, 0.3mg / L 6-benzylaminoadenine, 0.8mg / L naphthaleneacetic acid and 800mg / L hydrolyzed casein Cultivate in protein dedifferentiation medium for 5 days for dedifferentiation to form callus. The culture temperature is 25°C, the light time is 13h / d, and the light intensity is 50umol / m 2 s; (4) Transfer the callus to the redifferentiation medium including the medium mother solution, 0.8mg / L 6-benzylaminoadenine and 0.05mg / L naphthaleneacetic acid for 8 days, and the culture temperature is 25℃ , the light time is 13h / d, and the light intensity is 50umol / m 2 ·s.

Embodiment 3

[0017] A method for inducing embryogenic callus of Betula microphylla, the specific steps are as follows: (1) select rooted seedlings of Betula microphylla tissue-cultured with a seedling age of 35 days and a seedling height of 6 cm; (2) cut the stem section of the rooted plantlets cultured into (3) Inoculate the explants in a medium containing mother liquor, 0.5mg / L 6-benzylaminoadenine, 1.0mg / L naphthaleneacetic acid and 1000mg / L hydrolyzed casein Cultivate in protein dedifferentiation medium for 4 days to dedifferentiate and form callus. The culture temperature is 27°C, the light time is 14h / d, and the light intensity is 60umol / m 2 s; (4) Transfer the callus to the redifferentiation medium containing the medium mother solution, 1.0mg / L 6-benzylaminoadenine and 0.1mg / L naphthaleneacetic acid for 7 days, and the culture temperature is 27℃ , the light time is 14h / d, and the light intensity is 60umol / m 2 ·s.

[0018] In the above examples, the callus induction rate of the tis...

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PUM

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Abstract

The invention discloses a betula microphylla var.paludosa embryonic callus induction method, and specific steps are as follows: (1) selecting betula microphylla var.paludosa tissue culture rooting seedlings with the seedling age of 28-35d and the seedling height of 3- 6cm; (2) cutting stem sections of the tissue culture rooting seedlings into 0.2-0.3cm small segments to obtain explants (; 3) inoculating the explants into a dedifferentiation culture medium for culturing for 4-7d foe dedifferentiation to form callus, wherein the culture temperature is 23-27 DEG C, the illumination time is 12-14h / d, the light intensity is 40- 60 umol / m<2>. S; and (4) transferring the callus into a redifferentiation culture medium for culturing for 7-10d, wherein the culture temperature is 23-27 DEG C, the illumination time is 12-14h / d, and the light intensity is 40- 60 umol / m<2>. S; the betula microphylla var.paludosa embryonic callus induction method can provide a large number of embryogenic callus as a basic material for the betula microphylla var.paludosa mutant screening research, and can promote the research on betula microphylla var.paludosa breeding.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for inducing embryogenic callus of Betula marsh. Background technique [0002] Swamp betula is a new variety of Betula genus Betula in the Betaceae family. It is a unique species native to the lower reaches of the Neilang River in Altay County, Xinjiang. It is an extremely rare and endangered temperate deciduous broad-leaved tree species. Small deciduous trees or erect large shrubs with a height of 3 to 4 meters are resistant to cold, drought, and salt. They are excellent tree species for afforestation in saline-alkali land. [0003] At present, there have been reports on the introduction and cultivation of Betula microphylla, twig cutting propagation, and rapid propagation of seedlings using tissue culture technology. There is no report on the technology of inducing embryogenic cell generation using the stem of Betula microphylla test-tube plantlets as ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈权孙建肖建梅周羽崔心红朱义
Owner SHANGHAI SUNQIAO MODERN GREENHOUSE SEED & SEEDLING
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