Breeding method of radish cell nucleo-cytoplasmic interaction male sterile maintainer line

Abstract

The invention discloses a breeding method of a radish cell nucleo-cytoplasmic interaction male sterile maintainer line. According to the method, a maintainer line Goutouguan B of the radish cytoplastmale sterile maintainer line Goutouguan is used to be hybridized with fertile plant Shizhuangbai B in a Shizhuangbai two-type line after the castration; the hybridized first generation F1 group is obtained; sterile plant Shizhuangbai A in the Shizhuangbai two-type line is used as a female parent, and the hybridized first generation F1 group is used as a male parent for test crossing; meanwhile, the hybridized first generation F1 group is subjected to self crossing; the expression condition of the fertility of the test crossing later generations is observed; the later generations sterile test crossing combination is screened; the male parents of the later generations sterile test crossing combination are subjected to self crossing and are then subjected to back crossing with the Shizhuangbai B; through the test crossing for many times and the back crossing, a novel maintainer liner Shigou B is obtained; the cytoplasm of the maintainer line Shigou B contains the maintainer gene from theGoutouguan; the properties are similar to the original Shizhuangbai.

Description

Technical field [0001] The invention relates to the technical field of crop breeding, and more specifically to a method for breeding a radish cell nucleocytoplasmic male sterile maintainer line. Background technique [0002] Cruciferous plants are mostly cross-pollinated crops and have obvious heterosis. However, due to their small floral organs, manual emasculation is time-consuming and laborious, and few hybrid seeds can be obtained from one pollination. To solve this problem, breeding Workers select self-incompatible lines for breeding, but self-incompatible lines are susceptible to factors such as the environment, their compatibility is unstable, and the seed production purity is often not high. The use of plant male sterile lines for hybrid seed production has better solved the problem of self-incompatible seed production, and can improve the purity of hybrid seeds and increase the yield of crops. This has become the main development direction of the international seed indus...

AI Technical Summary

Problems solved by technology

However, in the study of radish, male sterile materials controlled by nuclear genes in natural populations are rarely reported. The ratio of each plant accounts for 50% ("Summary of Chinese Radish Male Sterility Research" Li Dechao "Yangtze River Vegetables" 2006 No. 6), when using it as the female parent for hybrid seed production, the sowing amount, sowing area, transplantation The planting area and planting density are more than doubled than when 100% male sterile lines are used for seed production, and in the early flowering stage, 50% of the pollen plants in the female parent row must be pulled out in time to maintain the purity of seed production. When there are powder plants in the female parent row, on the one hand, the remaining sterile plants will have uneven density in the field, causing lodging and grass shortage in the field, reducing seed production and affecting seed quality; Failure to remove pollen plants in time will cause pollen pollution and reduce the purity of hybrids

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