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Streptomyces albidoflavus and application thereof

A technology of Streptomyces lividans and culture, applied in application, bacteria, microorganisms and other directions, can solve the problem of no root-knot nematode control and other problems, and achieve the effects of inhibiting the growth of plant pathogens, good repair ability, and strong antagonism

Active Publication Date: 2017-11-07
陕西博秦生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no actinomycete preparation that can be used for root-knot nematode control in production.

Method used

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  • Streptomyces albidoflavus and application thereof
  • Streptomyces albidoflavus and application thereof
  • Streptomyces albidoflavus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Screening and Identification of Example 1 Bacterial Strains

[0034] 1. Test method:

[0035] 1.1 Isolation and screening: The strain was isolated and screened from the root zone soil of muskmelon healthy plants by Gao's No. 1 agar and dilution plate smearing method. The strain has disease resistance and growth promotion effect.

[0036] 1.2 Strain identification

[0037] Morphological characteristics: Colony morphology, single colonies were obtained by dilution plate smearing method, and the characteristics of colony shape, size, edge condition, uplift degree, surface gloss, viscosity and colony color were observed. Cell morphology: observation by scanning electron microscope. 16S rDNA sequence analysis After the sequence was obtained, the similarity search was carried out in the NCBI database through the Blast program, and the phylogenetic tree was constructed for the strain sequences with high homology by using the Neighbor-joining method in the Mega 5.0 software. ...

Embodiment 2

[0045] Example 2 Determination of growth-promoting properties of Streptomyces albidoflavus T4

[0046] 1. Test method

[0047] 1.1 Indoleacetic acid (IAA)-producing ability: under sterile conditions, pick a single colony of the Streptomyces albidoflavus T4 strain that has been activated on the plate and transfer it to Gaoshi No. 1 liquid medium, shake it at 30°C and 120rpm Cultivate for 8 days, remove bacteria from the fermentation broth by centrifugation, filter the obtained supernatant with a microporous membrane (0.22 μm) to obtain a cell-free fermentation filtrate, and store it in a refrigerator at 4°C for later use; use ethyl acetate as the extract The secondary metabolites in the T4 cell-free fermentation filtrate were extracted using a distillation device, and the secondary metabolites were distilled from the Streptomyces albidoflavus T4 cell-free fermentation filtrate extract, and the presence or absence of indole in the secondary metabolites was qualitatively analyzed...

Embodiment 3

[0055] Example 3 Determination of Nematicidal Action of Streptomyces albidoflavus T4

[0056] 1. Test method

[0057] 1.1 Preparation of Streptomyces albidoflavusT4 fermentation broth: under sterile conditions, pick a single colony of Streptomyces albidoflavusT4 strain that has been activated on the plate and transfer it to Gaoshi No. 1 liquid medium, and culture it on a shaking table at 30°C and 120rpm for 8 days The fermentation broth was removed by centrifugation to remove bacteria, and the obtained supernatant was sterilized by suction filtration with a microporous membrane (0.22 μm) to obtain a cell-free fermentation filtrate, which was stored in a refrigerator at 4°C for later use.

[0058] 1.2 Preparation of Caenorhabditis elegans suspension: Inject 5 mL of sterile water into the culture dish overgrown with nematodes, shake it for 30 seconds, place it on an inclined plane for 2 minutes, collect and enrich the sterile water containing a large amount of Caenorhabditis ele...

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Abstract

The invention belongs to the technical field of plant pest biological prevention and control, and particularly discloses streptomyces albidoflavus T4, which is preserved in the China Center for Type Culture Collection on May 4, 2017, and has a preservation number of CCTCC M 2017237. The invention further discloses application of the strain in prevention and control of plant pests, soil-borne root diseases of crops and nematodes, and application of the strain in crop seed vitality improvement. The streptomyces albidoflavus T4 can be used for producing plant growth promoting substance heteroauxin and plant growth promoting biomass iron carrier, so that the root growth of crops can be remarkably promoted, the crop root vitality can be reinforced, and the crop seed vitality is remarkably improved. The streptomyces albidoflavus has an excellent repairing capability for successive cropping obstacles including root-knot nematodes and root soil-borne diseases.

Description

technical field [0001] The invention relates to the technical field of biological control of plant diseases and insect pests, in particular to a strain of Streptomyces microalbiflous and application thereof. Background technique [0002] The obstacle of continuous cropping has become a restrictive factor affecting the development of protected horticulture and specialized planting industries. Root-knot nematode disease caused by continuous cropping is a form of continuous cropping failure, which can cause economic losses of more than 100 billion US dollars per year worldwide. Although chemical pesticides can alleviate root-knot nematode disease to a certain extent, their residues in agricultural products endanger food safety and cause environmental pollution in soil, which limits the application of chemical nematicides in production. [0003] The pollution-free microbial control is one of the main ways to solve the obstacles of plant continuous cropping. Screening biocontro...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P3/00A01N63/00A01P3/00A01P5/00A01P21/00C12P17/10C12R1/465
CPCA01N63/00C12P3/00C12P17/10C12N1/205C12R2001/465
Inventor 马媛媛薛泉宏来航线
Owner 陕西博秦生物工程有限公司
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