Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Blood sugar reducing effective parts and components of Dendrobium chrysanthum as well as preparation methods and applications of effective parts and components

A technology of Dendrobium bougainvillea and effective parts, which is applied in the field of pharmaceuticals, can solve the problems that there are no research reports on Dendrobium bougainvillea and its preparations, and achieve the effects of improving insulin sensitivity, promoting consumption, and expanding medicinal resources

Inactive Publication Date: 2017-11-07
GUANGDONG PHARMA UNIV
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the existing research, there is no research report on the use of Dendrobium bougainvillea and its preparations in the prevention and treatment of glucose and lipid metabolism disorders

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Blood sugar reducing effective parts and components of Dendrobium chrysanthum as well as preparation methods and applications of effective parts and components
  • Blood sugar reducing effective parts and components of Dendrobium chrysanthum as well as preparation methods and applications of effective parts and components
  • Blood sugar reducing effective parts and components of Dendrobium chrysanthum as well as preparation methods and applications of effective parts and components

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of the total extract of Dendrobium spp.: grind 7.5 kg of dried stems of D. spp., extract with 95% ethanol under reflux for 4 times, each time for 3 hours, filter with suction, and recover the solvent by rotary evaporation to obtain 550 g of total extract of D. spp.

[0029] The total extract of Dendrobium bougainvillea (high and low dose groups) was acted on type 2 diabetic rats (induced by high-sugar and high-fat feeding combined with small-dose injection of STZ), by comparing the changes in blood sugar and body weight of the rats before and after administration, and determining After administration, the four items of blood lipids, insulin content and blood sugar level in rats were combined with oral glucose tolerance test to study the effect of Dendrobium bougainvillea on type 2 diabetic rats.

[0030] The results showed that compared with the blood sugar level of rats before administration, the high-dose group of Dendrobium candidum can significantly reduc...

Embodiment 2

[0078] Preparation of the effective hypoglycemic fraction of Dendrobium bougainvillea: the total extract obtained in Example 1 was dissolved in 2 times the volume of water, and extracted successively with petroleum ether, chloroform, ethyl acetate and n-butanol to obtain four extracts respectively. The extract of the part; finally, the extract of each extraction part is concentrated and evaporated to dryness respectively to obtain effective parts of petroleum ether, effective parts of chloroform, effective parts of ethyl acetate and effective parts of n-butanol.

[0079] Insulin-resistant liver cancer cells (HepG2) induced by high concentrations of insulin were used to test the activity of improving insulin resistance of each part of the extract, and the comprehensive results of cell glucose consumption and MTT screening showed that the insulin sensitivity of HepG2 cells in the chloroform administration group was significantly improved. Improvement, when the dosing concentratio...

Embodiment 3

[0082] The chloroform part obtained in Example 2 was subjected to 200-300 mesh silica gel column chromatography, using silica gel as an adsorbent, petroleum ether-dichloromethane, dichloromethane-methanol for gradient elution, each 1000mL as a fraction, Petroleum ether: dichloromethane = 100:1, 20:1, 10:1, 8:1, 6:1, 5:1, 4:1, 3:1, 2:1, 1:1 and dichloromethane 11 gradients of methane for the first stage elution, and then dichloromethane: methanol = 100:1, 50:1, 20:1, 10:1, 5:1, 4:1, 2:1 and pure methanol 8 A gradient for the second stage elution; dichloromethane:methanol=50:1 fractionated through 200-300 mesh silica gel column chromatography, Sephadex LH-20 column chromatography and petroleum ether or ethyl acetate recrystallization separation and purification to obtain the compound isocyanate Neotigenin and 26-O-Neutigenin glucoside.

[0083] Compound 1: Isonentinegenin; Compound 2: The monomeric hypoglycemic moiety of 26-O-Neutigenin glucoside.

[0084] Table 4 compound 1, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention proposes a blood-sugar-lowering effective part of Dendrobium bougainvillea. The effective part is dried and pulverized stems of Dendrobium bougainvillea, then refluxed with ethanol solution to extract, and suction-filtered the extract to obtain the total extract; the total extract is dissolved in water, successively Different solvents are used for extraction to obtain extracts of four extraction parts; finally, the extracts of each extraction part are concentrated and evaporated to dryness to obtain four polar parts. The effective hypoglycemic part: Part B was separated and purified to obtain compounds with unique structures, isoneeutinin and 26-O-neutigenin glucoside, which were found to be active ingredients by hypoglycemic tests. The present invention also proposes the application of the above effective parts and active ingredients. The extracts from various parts of the Dendrobium bougainvillea of ​​the invention can promote the consumption of glucose by insulin-resistant HepG2 cells. Isonecinogenin and 26‑O‑Neutygenin glucoside restore insulin regulation of blood sugar by improving insulin resistance.

Description

technical field [0001] The invention relates to the technical field of pharmacy, in particular to an effective part and active ingredient of Dendrobium bougainvillea for lowering blood sugar, and a preparation method and application thereof. Background technique [0002] Dendrobium, one of the Orchidaceae plants, is mainly distributed in Southwest China, South China, Taiwan and other places in my country. It has curative effects on the human body such as distorting deficiency heat, benefiting essence and strengthening yin. Its medicinal value has been recognized by the world and is widely used. Dendrobium was first recorded in "Shen Nong's Materia Medica", which records: "It is sweet, flat, and non-toxic; in curing injuries, it can remove numbness, lower qi, nourish the five viscera and fatigue, win thinness, and strengthen yin; long-term use can thicken the stomach, lighten the body, Prolong life, grow muscles, expel evil heat from the skin, resolve shocks with determinatio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8984A61P3/10C07J71/00A61K31/7048A61K31/58A23L33/105A61K135/00
CPCA61K36/8984A23L33/105A23V2002/00A61K31/58A61K31/7048A61K2236/331A61K2236/333A61K2236/39A61K2236/51A61K2236/55C07J71/0005
Inventor 蔡金艳
Owner GUANGDONG PHARMA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com