Method for preparing high-activity ginsenoside by solid dynamic fermentation technology

Abstract

The invention discloses a method for preparing high-activity ginsenoside by a solid dynamic fermentation technology and particularly relates to a high-activity ginseng product prepared by using plant tissues of panax species as a solid culture medium, inoculating with a single aerobic strain which is selected and can generate ginsenoside hydrolase and carrying out dynamic fermentation and rich in C-K and F2. The ginseng product is extracted and separated to obtain a C-K and F2 mixed saponin or monomer saponin; or a mixture or a monomer of the C-K, Rh2 and diol saponins can be further prepared by using the hydrolase extracted from the high-activity ginseng product. According to the method, high-activity underglycosylated ginsenoside can be obtained by directly using the plant tissues of the panax species as a raw material for fermenting without preparation of original diol saponin PPD (Paraphenylenediamine) or special preparation of panaxsaponin glucosidase. The method has the advantages of few process steps, low production cost, no pollution and suitability for industrial production.

Description

technical field [0001] The invention relates to the field of ginsenosides, in particular to a method for preparing highly active ginsenosides through solid dynamic fermentation technology. Background technique [0002] The ginseng plants grown in my country are mainly ginseng from Northeast China, Sanqi ginseng from Yunnan and Guangxi, and American ginseng from Shandong and Jilin. There are also a small amount of bamboo ginseng and pearl ginseng being planted and used. Its main activity depends on the saponins. Saponin is a kind of glycoside connected by glycoside and non-sugar ligand through glycosidic bond. According to the difference between glycosyl and ligand and the position of glycosidic bond, saponin is divided into different types. Different types of saponins are physically and The chemical properties are different. Can be divided into panaxadiol saponins (PPD) according to the difference of structure, such as Rb1, Rb2, Rb3, Rc, Rd, Rg3, Rg5, Rh2, Rh3, F2, C-K etc...

AI Technical Summary

Problems solved by technology

However, due to the use of complex bacteria for fermentation, the optimal culture conditions for each type of bacteria are different, and it is also difficult to ensure that the number ratio of each type of bacteria is consistent in each culture process, which greatly increases the difficulty of the culture process and the fermentation products are uncertain. , many by-products, low conversion rate of low glycosyl saponins, etc.

It is also difficult to separate low-glycoside saponins or monomers from the fermentation product, and the patent does not provide a relevant solution

[0027] In summary, the reported methods of biotransformation of ginsenosides to prepare rare saponins have disadvantages such as high production cost, low conversion rate, large volume of fermentation broth, cumbersome process, complicated operation, and difficult separation and purification.

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