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Preparation method of traditional Chinese medicinal probiotic fermentation metabolite for preventing and treating bacterial diarrhea of piglets

A metabolite, bacterial technology, applied in the field of probiotic metabolites

Pending Publication Date: 2017-10-27
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that there is no combination of various probiotics producing antimicrobial peptides and natural anti-inflammatory Chinese medicines to prevent and treat piglet bacterial diarrhea, and to provide a traditional Chinese medicine probiotic for the prevention and treatment of piglet bacterial diarrhea. The method of preparation of the product

Method used

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  • Preparation method of traditional Chinese medicinal probiotic fermentation metabolite for preventing and treating bacterial diarrhea of piglets
  • Preparation method of traditional Chinese medicinal probiotic fermentation metabolite for preventing and treating bacterial diarrhea of piglets
  • Preparation method of traditional Chinese medicinal probiotic fermentation metabolite for preventing and treating bacterial diarrhea of piglets

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specific Embodiment approach 1

[0018] Specific embodiment one: a kind of preparation method of the traditional Chinese medicine probiotic fermentation metabolite of preventing and treating piglet bacterial diarrhea of ​​the present embodiment, it is carried out according to the following steps:

[0019] 1. Preparation of fermentation medium: crush soybean meal, corn flour, Radix Isatidis, Dandelion, Coptis chinensis, Folium Folium and Flos Lonicerae into 150 mesh particles, according to the mass ratio of soybean meal, corn flour, Isatis Radix, dandelion, Coptis chinensis, Folium Folium Folium and Flos Lonicerae 2-2.5: 5-6: 0.4-0.5: 0.4-0.5: 0.4-0.5: 0.35-0.4: 0.25-0.3 to obtain dry fermentation medium powder;

[0020] 2. Sterilization of the fermentation medium by heating: add water to the dry powder of the fermentation medium to prepare a fermentation medium with a concentration of 10% to 15% by mass; sterilize in situ in the fermentation tank, and sterilize at a constant temperature of 80 to 90°C for 3.5 t...

specific Embodiment approach 2

[0024] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the mass ratio of soybean meal, corn flour, Radix Radix, Dandelion, Coptis chinensis, Daqingye and honeysuckle in the dry powder of the fermentation medium in step 1 is 2:6:0.5 :0.5:0.4:0.35:0.25. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0025] Embodiment 3: The difference between this embodiment and Embodiment 1 is that the mass ratio of soybean meal, corn flour, Radix Radix, Dandelion, Coptis chinensis, Folium Folium and Flos Lonicerae in the dry powder of the fermentation medium in step 1 is 2:5:0.4 :0.4:0.4:0.35:0.25. Others are the same as in the first embodiment.

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Abstract

The invention discloses a preparation method of a traditional Chinese medicinal probiotic fermentation metabolite for preventing and treating bacterial diarrhea of piglets and relates to the field of prevention and treatment of bacterial diarrhea of piglets. The method comprises the following steps: (1) preparation of a fermentation medium, namely mixing soybean meal, corn flour and a natural anti-inflammation traditional Chinese medicine at a certain ratio to prepare the fermentation medium; (2) biological enzymolysis of thefermentation medium; (3) multi-bacterial synergetic liquid fermentation, namely inoculating lactobacillus casei into the fermentation medium, further inoculating enterococcus faecium for fermental cultivation, and then inoculating bacillus licheniformis for fermental cultivation; (4) solid secondary fermentation; and (5) low-temperature drying. The total viable bacteria of a product reach 3*10<10>CFU / g; an in vitro test proves that escherichia coli and salmonella bacterium have a significant inhibition effect; piglet feeding proves that the recovery rate of the product on the bacterial diarrhea of the piglets reaches 87.6%, the direct cost of the product is 7,000-10,000 per ton, the market price is 50,000-100,000 per ton and the traditional Chinese medicinal probiotic fermentation metabolite has relatively high additional value.

Description

technical field [0001] The invention relates to the field of preventing and treating bacterial diarrhea of ​​piglets. It specifically relates to a method that uses soybean meal, corn flour and natural anti-inflammatory traditional Chinese medicine as raw materials, and utilizes a variety of antibacterial peptide-producing probiotics for collaborative fermentation to obtain probiotic metabolites rich in various antibacterial peptides and anti-inflammatory active ingredients of traditional Chinese medicine, as feed Additives to prevent bacterial diarrhea in piglets. Background technique [0002] Probiotics can produce antibacterial peptides and other antibacterial active substances, which can effectively kill harmful intestinal microorganisms such as Escherichia coli, Salmonella, etc., and prevent and treat bacterial diseases of the digestive tract such as diarrhea. The most effective probiotics are bacillus and lactic acid bacteria. Bacillus subtilis has a wide range of bac...

Claims

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Application Information

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IPC IPC(8): A23K50/30A23K10/30A23K10/12A23K10/37A61K36/718A61K36/855A61P1/12A61P31/04C12N1/20C12R1/245C12R1/46
CPCA23K10/12A23K10/30A23K10/37A23K50/30A61K36/195A61K36/288A61K36/315A61K36/355A61K36/704A61K36/718A61K36/855A61K2236/15A61K2236/17A61K2236/19C12N1/20A61K2300/00Y02P60/87
Inventor 敖金霞高学军刘营甄贞孙喆
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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