Rapid-recognition gene chip for pathogenic bacteria of pneumonia

Abstract

The invention discloses a rapid-recognition gene chip for pathogenic bacteria of pneumonia. The gene chip can detect 15 pathogenic bacteria including streptococcus pneumoniae, staphylococcus aureus, haemophilus influenzae, mycoplasma pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, enterococcus faecalis, enterococcus faecium, klebsiella pneumoniae, escherichia coli, enterobacter cloacae, stenotrophomonas maltophilia, burkholderia cepacia, legionella pneumophila and chlamydia pneumoniae, and clinically common and difficult-to-culture pathogenic bacteria are contained. In a preparation process of the gene chip, design, screening and verification of probes are performed by adopting 16S rDNA and a specific gene sequence corresponding to each of the pathogenic bacteria, and types of the bacteria in a to-be-detected sample are identified from levels of genus and species simultaneously and respectively. The gene chip has the advantages that the defect that clinical detection of the pathogenic bacteria of pneumonia is not timely and comprehensive at present is overcome, and one novel detection way is provided for early diagnosis and early treatment of patients with pneumonia.

Description

technical field [0001] The invention relates to gene technology, in particular to a gene chip for rapid identification of pneumonia pathogenic bacteria. Background technique [0002] In recent years, due to the aggravation of air pollution and the occurrence of public health incidents, respiratory diseases have attracted more and more attention. Pneumonia refers to the inflammation of lung parenchyma including terminal airways, alveoli and interstitium, and is the leading cause of death from infectious diseases worldwide [1] . According to the World Health Organization (WHO), about 3.5 million people die from lower respiratory tract infection every year, ranking third among all causes of death. [2] . Pneumonia and influenza are the ninth leading cause of death in the United States, with more than 50,000 deaths from pneumonia in both 2010 and 2011 [3-5] , and the statistics did not include the cases of sepsis caused by pneumonia and cancer and Parkinson's who died of pneu...

AI Technical Summary

Problems solved by technology

The detection of bacterial serological antibodies is a rapid detection method, but different antibodies are produced at different times. For example, Mycoplasma pneumoniae IgM antibodies are produced about 2 weeks after the onset of the disease, which cannot meet the needs of rapid clinical diagnosis, and the sensitivity and specificity Unsatisfactory

Infectious Diseases Society of America (IDSA) guidelines once recommended giving antibiotics to patients with lung infections within 8 hours, which was shortened to 4 hours in 2003 [19,20] , it can be seen that the culture, isolation and identification of bacteria is far from meeting the needs of clinical rapid diagnosis, and the early treatment of pneumonia can only rely on empirical treatment, which further aggravates the emergence of drug-resistant strains. Therefore, an accurate and rapid detection method is urgently needed clinically. Pneumonia Pathogen Method

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