Extract of Rhus typhina fruit and preparation method thereof, and application of extract to fermentation production of genetic engineering products

Abstract

The invention provides the extract of Rhus typhina fruit and a preparation method thereof, and application of the extract to fermentation production of genetic engineering products, belonging to the technical field of processing of agricultural products. The method comprises the following steps: mixing dried Rhus typhina fruit with an extraction solution so as to obtain a mixed material, wherein the extraction solution is an aqueous solution of ethanol; subjecting the mixed material to heating reflux under a slight boiling state, then carrying out cooling to room temperature and carrying out filtering and centrifugation so as to obtain clear liquid; and subjecting the clear liquid to pressure-reduced concentration according to a volume ratio of 8-12: 1 and carrying out degerming so as to obtain the extract of Rhus typhina fruit, i.e., RtSE. The extract RtSE can increase the amount of host bacteria in a genetic engineering fermentation system; when only RtSE is used in the fermentation system, the biomass of host cells in the fermentation system is increased by 5% or above, and a total protein amount and the active expression quantity of exogenous proteins are both increased by 20% or above; and when RtSE and IPTG are both used in the fermentation system, the biomass of the host cells in the fermentation system is increased by 12% or above, the total protein amount is increased by 30% or above, and the active expression quantity of the exogenous proteins are increased by 35% or above.

Description

technical field [0001] The invention belongs to the technical field of agricultural product processing, and in particular relates to a torch tree fruit extract (extraction from Rhus Typhina, RtSE), a preparation method and the application of the extract in the fermentation production of genetic engineering products. Background technique [0002] Torch tree (Rhus hirta L.), a Anacardiaceae plant, is a deciduous shrub native to North America, mainly distributed in tropical and subtropical regions. It was introduced to my country by the Institute of Botany, Chinese Academy of Sciences in 1959, and has been promoted to all provinces and regions of the country since 1974. It is more cultivated in the provinces (regions) north of the Yellow River Basin. Torch tree fruit can be used not only as a dietary condiment, but also made into beverages or other medicines. It has been reported that the extract of torch tree fruit can be anti-cancer, anti-inflammatory, and even has the effects...

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Problems solved by technology

[0005] In order to overcome the adverse effects of IPTG, methods such as temperature-induced lac system and lactose-induced lac system have been developed and applied by different research groups, but temperature induction requires large energy consumption and high cost for industrial large-scale fermentation (MurrayNE.et al.J Molec Biol, 1979 132:493~505.); when lactose is induced, lactose has many influences on the proliferation and physiological behavior of the host as the carbon source of bacteria, and the quality control of genetic engineering products is difficult (Beckwith JR.1987.Cellularand Molecular Biology. American Society for Microbioloty, Washington, DC. Vol 2, pp1444~1452.)

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