Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of semen aesculi extract to preparation of medicine for preventing and treating senile dementia

A technology of sala seeds and extracts, applied in the field of traditional Chinese medicine, can solve problems such as obvious adverse reactions, easy formation of drug resistance, and inability to alleviate the development of diseases

Inactive Publication Date: 2017-10-24
南京惠宝生物医药有限公司
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above drugs can improve the symptoms of AD patients in the short term, but cannot alleviate the development of the disease, and are prone to drug resistance, with obvious adverse reactions; while traditional Chinese medicine has good clinical effects in the prevention and treatment of mild cognitive impairment and senile dementia. The characteristics of pathways and multi-link overall regulation, and the use of natural medicines has less toxic and side effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of semen aesculi extract to preparation of medicine for preventing and treating senile dementia
  • Application of semen aesculi extract to preparation of medicine for preventing and treating senile dementia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: In vitro studies have shown that the extracts of Sala chinensis can inhibit the neurotoxicity of Aβ and have a therapeutic effect on AD

[0017] 1. Cell lines and reagents

[0018] Rat pheochromocytoma SH-SY5Y cells (purchased from ATCC), DMEM / F12, fetal bovine serum, MTT, donepezil hydrochloride (purchased from Sigma, USA), huperzine A (purchased from Sigma, USA), sodium aescin (Shandong Luye Pharmaceutical Co., Ltd.)

[0019] 2. Drug preparation

[0020] ① Sodium aescinate was dissolved in DMSO and diluted with normal saline to prepare concentrations of 1 mg / mL, 5 mg / mL, and 10 mg / mL

[0021] ② Aβ25-35 was prepared to 100 μmol / L with triple distilled water, filtered, aliquoted, and stored at -20°C; prepared to the required concentration before use, and incubated at 37°C for 7 days.

[0022] ③Positive drug: Donepezil hydrochloride and huperzine A were dissolved in blank serum, the concentration of donepezil hydrochloride was 5 mg / ml, and huperzine A was 0...

Embodiment 2

[0036] Example 2: In vivo study proves that the extract of Sala chinensis has therapeutic effect on APP / PS1 double transgenic mice with dementia

[0037] 1 material

[0038] 1.1 Experimental animals

[0039] Forty-eight 5-month-old APP / PS1 double transgenic mice, male, weighing 25±2g, SPF grade, and 8 C57BL / 6J mice with the same genetic background were normal controls.

[0040] 1.2 Experimental drugs and reagents

[0041] The positive control drug is donepezil hydrochloride (trade name: Aricept), 5 mg / tablet, the tablet is ground into powder before use, and double-distilled water is used to make a drug solution for oral administration of 0.65 mg / kg d - 1 ; Huperzine A Capsules were prepared with double distilled water to make a drug solution for gastric administration, and the prepared concentration was 0.026mg / kg·d - 1 . Acetylcholinesterase (AChE) kit, choline acetyltransferase (ChAT) kit, SOD activity kit, MDA content kit, GSH content kit. The following "effective compon...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of a semen aesculi extract to preparation of a medicine and a health-care product for preventing and treating senile dementia, wherein the active component of the semen aesculi extract is beta-aescin. According to experiments, the traditional Chinese medicine semen aesculi extract can obviously improve dysmnesia of model animals and improve the learning capability, can effectively improve and adjust central cholinergic nerve as well as adjust free radical metabolism, and has exact curative effect on various kinds of senile dementia such as Alzheimer's disease, vascular dementia and mixed dementia.

Description

technical field [0001] The present invention relates to the preparation method and application of the extract of saffron seeds, in particular to the application of the extract of saffron seeds in the prevention or treatment of Alzheimer's disease, which belongs to the field of traditional Chinese medicine. Background technique [0002] Senile dementia mainly includes Alzheimer's disease (AD) and vascular dementia (VD). Alzheimer's disease accounts for about 60-70%, and vascular dementia accounts for about 20-30%. Alzheimer's disease (AD) is a degenerative disease of the central nervous system mainly characterized by progressive dementia. Its clinical features are memory and other cognitive dysfunction, and early clinical symptoms include motor, sensory or coordination deficits. The main pathological changes in AD patients are senile plaques formed by extracellular amyloid deposition, neurofibrillary tangles, and loss of extensive neuronal synapses, inflammation, oxidation a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/704A61P25/28A23L33/105A23L2/52A23F3/00
CPCA23F3/00A23L2/52A23V2002/00A61K31/704A61K36/185A23V2200/30A23V2200/322A23V2250/21A23V2250/5118A23V2250/5114A23V2250/612A23V2250/51084A23V2250/51086A23V2250/6422A23V2250/6414A23V2250/61A23V2250/6418A23V2250/0622
Inventor 不公告发明人
Owner 南京惠宝生物医药有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com