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Application of metformin in preparation of drug for cervical cancer

A metformin and cervical cancer technology, applied in the field of metformin in the preparation of cervical cancer drugs, can solve the problems of high recurrence rate, serious adverse reactions, HPV vaccine treatment, etc.

Inactive Publication Date: 2017-10-24
FOSHAN MATERNAL & CHILD HEALTH CARE HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the deficiencies of the above-mentioned prior art, the purpose of the present invention is to provide a kind of metformin with little side effect, low price and good development prospects in the preparation of medicines for cervical cancer, aiming to solve the problem of cervical cancer in the prior art. In terms of treatment, the traditional surgical treatment has a high recurrence rate after surgery, and the combination of chemotherapy and surgery is likely to cause serious adverse reactions, and there are technical problems with obvious limitations in HPV vaccine treatment.

Method used

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  • Application of metformin in preparation of drug for cervical cancer
  • Application of metformin in preparation of drug for cervical cancer
  • Application of metformin in preparation of drug for cervical cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Preparation of Metformin Hydrochloride Solution and Administration to Animals

[0039] Metformin hydrochloride (purchased from Sigma-aldrich company, chemical abstract registration number: 1115-70-4) was dissolved in distilled water to prepare different concentrations (200mM, 100mM, 50mM, 25mM, 20mM, 12.5mM, 10mM, 6.25 mM, 3.13mM, 2mM, 0mM) metformin hydrochloride solution, set aside.

[0040] Metformin hydrochloride was prepared into 50 mg / kg and 250 mg / kg metformin hydrochloride solutions with distilled water, and the distilled water negative control group was administered by intraperitoneal injection.

Embodiment 2

[0041] Example 2 Metformin inhibits the proliferation of cervical cancer Hela and Caski cells

[0042] Cervical cancer cell lines HeLa and CasKi (purchased from China Atypical Collection Center) in logarithmic growth phase were seeded in 96-well plates at a density of 1000-2000 cells / well, and replaced with different concentrations of ( 200mM, 100mM, 50mM, 25mM, 12.5mM, 6.25mM, 3.13mM, 0mM) Metformin complete medium, respectively at different time points (24 hours, 48 ​​hours and 96 hours), by XTT method (XTT, 3,3 '-[1-(phenylaminoyl)-3,4-tetrazolium]-bis(4-methoxy-6-nitro)sodium benzenesulfonate, purchased from Sigma; XTT method, cell viability detection method ) to detect cell proliferation. For the detection method, see "Experimental Techniques of Medical Immunology" (Soochow University Press, publication date: February 2011).

[0043] Such as figure 1 As shown, as the concentration increases, the inhibitory effect of metformin on cell proliferation increases, and its IC5...

Embodiment 3

[0044] Example 3 Metformin promotes apoptosis of cervical cancer cells

[0045] The cervical cancer cell lines HeLa and CasKi (purchased from China Atypical Collection Center) in the logarithmic growth phase were washed twice with PBS (phosphate buffered solution, phosphate buffered saline) (2000rpm, centrifuged for 5min); the cells were collected , add 500µL binding buffer to suspend cells; then add 500µL Annexin V-FITC and mix well, then add 5µL Propidium Iodide (propidium iodide), mix well; In the case of death, the excitation wavelength Ex=488 nm, the emission wavelength Em=530nm, the green fluorescence of Annexin V-FITC is detected through the FITC channel (FL1); the PI red fluorescence is detected through the PI channel (FL3). After adding 2 µg / mL PI and co-bathing with the medium for 1 h, observe the absorption capacity of PI after cell membrane damage with an inverted fluorescence microscope, then treat the cells with methanol and acetone (1:1) for 5 min, wash with PBS...

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Abstract

The invention discloses application of metformin in preparation of a drug for cervical cancer. Metformin hydrochloride or a composition of the metformin hydrochloride serves as the drug, and by inhibiting PI3K and up-regulating p53 and p21 protein expression, the drug inhibits the proliferation of cervical cancer cells, promotes the apoptosis of the cervical cancer cells, and induces cervical cancer cell cycle arrest to realize prevention and treatment of the cervical cancer. The side effect of the metformin is smaller than that of first-line drugs for the cervical cancer such as platinum drugs, the price of the metformin is lower than that of HPV (human papillomavirus) vaccine, and the metformin has a great anti-cervical cancer potential and a good development prospect.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to the application of metformin in the preparation of cervical cancer medicine. Background technique [0002] Cervical cancer is one of the most common tumors in women all over the world. Its incidence rate is second only to breast cancer, lung cancer, and rectal cancer, ranking fourth among female tumor diseases. According to conservative estimates, there are about 500,000 new clinically diagnosed cases of cervical cancer in the world every year, most of which occur in developing countries. With the popularity of cervical screening, the incidence of cervical cancer in my country has dropped from 14.6 / 100,000 to 4.3 / 100,000 in the past 20 years, but in some rural areas with poor medical services, cervical cancer still has a high mortality rate And there is a trend of rejuvenation. The traditional treatment methods for cervical cancer mainly include surgery, chemotherapy ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/155A61P35/00
CPCA61K31/155
Inventor 夏承来
Owner FOSHAN MATERNAL & CHILD HEALTH CARE HOSPITAL
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