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Compound microbial preparation and preparation method thereof

A technology of composite microorganisms and preparations, which is applied in the field of environmental biology, can solve the problems of low biological treatment efficiency, and achieve the effects of simple operation, broad application prospects and high treatment efficiency

Inactive Publication Date: 2017-10-20
GUANGDONG PROVINCIAL ACADEMY OF ENVIRONMENTAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of compound microorganism preparation and preparation method thereof for the treatment of high salinity organic waste water in order to provide a kind of compound microorganism preparation and preparation method thereof for the low efficiency of the biological treatment of high salinity organic waste water in the prior art, and this preparation can be used for soy sauce Production, fermented bean curd production, meat processing and other wastewater treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: soy sauce industrial wastewater treatment

[0027] A soy sauce factory in Guangdong produces 1,500 cubic meters of waste water per day, with a salt content of 8,000-9,000 mg / L, COD Cr 2000-3000mg / L, ammonia nitrogen 50-70mg / L.

[0028] Inoculate Bacillus amyloliquefaciens CGMCC1.7463, Halomonas halophilus CGMCC1.9009, Mycobacterium salidenizima MCCC1B00742 and Pseudomonas fluorescens CGMCC1.6279 on the corresponding medium respectively, and expand the culture at 25-30°C . The concentration of Bacillus amyloliquefaciens CGMCC1.7463 expanded to 1.3×10 8 CFU / mL, the concentration after expanding the culture of Halomonas halophilus CGMCC1.9009 was 1.5×10 8 CFU / mL, the concentration after expanding culture of Mycobacterium salidenitrite MCCC1B00742 is 2×10 8 CFU / mL, the concentration of Pseudomonas fluorescens CGMCC1.6279 expanded culture was 8×10 8 CFU / mL.

[0029] The fermented products of Bacillus amyloliquefaciens CGMCC1.7463, Halomonas halophilus CGM...

Embodiment 2

[0031] Example 2: Seafood Processing Wastewater Treatment

[0032]Wastewater from a seafood processing plant contains organic matter such as protein and colloids, COD Cr 600-800mg / L, ammonia nitrogen 70-90mg / L, seawater is used as a pretreatment process in the processing engineering, so the chloride ion content in wastewater is as high as 4000-5000mg / L.

[0033] Inoculate Bacillus amyloliquefaciens CGMCC1.7463, Halomonas halophilus CGMCC1.9009, Mycobacterium salidenizima MCCC1B00742 and Pseudomonas fluorescens CGMCC1.6279 on the corresponding medium respectively, and expand the culture at 25-30°C . The concentration of Bacillus amyloliquefaciens CGMCC1.7463 expanded to 1×10 8 CFU / mL, the concentration after expanding the culture of Halomonas halophilus CGMCC1.9009 was 2×10 8 CFU / mL, the concentration after expanding culture of Mycobacterium salidenitrite MCCC1B00742 is 5×10 8 CFU / mL, the concentration of Pseudomonas fluorescens CGMCC1.6279 expanded culture was 6×10 8 CFU / ...

Embodiment 3

[0036] Embodiment 3: treatment of pickling waste water in pickled mustard factory

[0037] The wastewater of a mustard factory sewage treatment station mainly comes from pickling wastewater, washing water, desalinated water, pressing dehydration, etc. intermittently discharged during the mustard production process, as well as some domestic sewage. COD Cr 2000mg / L, ammonia nitrogen 60mg / L, salt content 5000mg / L.

[0038] Inoculate Bacillus amyloliquefaciens CGMCC1.7463, Halomonas halophilus CGMCC1.9009, Mycobacterium salidenizima MCCC1B00742 and Pseudomonas fluorescens CGMCC1.6279 on the corresponding medium respectively, and expand the culture at 25-30°C . The concentration of Bacillus amyloliquefaciens CGMCC1.7463 expanded to 5×10 8 CFU / mL, the concentration after expanding the culture of Halomonas halophilus CGMCC1.9009 was 5×10 8 CFU / mL, the concentration of Mycobacillus salidenitrophilus MCCC1B00742 expanded culture was 1.5×10 8 CFU / mL, the concentration of Pseudomon...

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Abstract

The invention discloses a compound microbial preparation and a preparation method thereof. The compound microbial preparation contains bacillus amyloliquefaciens CGMCC1.7463, halomonas halophila CGMCC1.9009, virgibacillus halodenitrificans and pseudonocardia ammonioxydans MCCC1B00742 and pseudomonas fluorescens CGMCC1.6279 and is prepared after the four bacteria are subjected to enlarged culture and fermented products are uniformly mixed in a certain proportion. The compound microbial preparation can be directly applied to treatment of wastewater containing high-concentration salt substances and organic matter, has the removal rate of CODCr up to 50%-90% and the removal rate of ammonia nitrogen up to 40%-80%, has the advantages of being simple to operate and resource-saving, can be widely applied to biological treatment of high-salinity organic wastewater and has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of environmental biology, and in particular relates to a compound microbial preparation and a preparation method thereof. Background technique [0002] The wastewater produced by soy sauce production, fermented bean curd production, meat processing and other enterprises contains not only high concentration of organic matter, but also high concentration of salt substances. Using technologies such as electrolysis and membrane separation, because the wastewater is not pure brine, there are many other organic impurities, which affect the treatment effect, and the operating cost is high. Using traditional biological methods for treatment, high-concentration salts have an inhibitory effect on microorganisms. If dilution + biochemical treatment is used, it is theoretically feasible, but this will cause waste of water resources, make the treatment facilities huge, increase investment, and increase operating costs. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07C12R1/39C12R1/01C02F103/32
CPCC02F3/341C02F2103/32C12N1/20
Inventor 王开演罗育池黄志华汪永红李朝晖叶向东黄振雄杨佘维
Owner GUANGDONG PROVINCIAL ACADEMY OF ENVIRONMENTAL SCI
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