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Preparation method of multilayer assembled micron-grade mono-disperse polystyrene magnetic composite microspheres

A technology of polystyrene microspheres and magnetic composite microspheres, which is applied in the field of preparation of multilayer assembled micron-scale monodisperse polystyrene magnetic composite Problems such as wide particle size distribution of polymer microspheres

Active Publication Date: 2017-10-13
成都爱兴生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the microspheres obtained by the existing method have a wide particle size distribution, which is not suitable for a wide range of applications in biomedicine, and monodispersity is an important indicator for measuring polymer magnetic microspheres, especially when applied to chemiluminescence immunology, In the field of cell separation and protein purification
[0004] Based on the above related background, the preparation methods of magnetic polymer microspheres in this field generally have the disadvantages that the prepared magnetic polymer microspheres have a wide particle size distribution, do not have monodispersity, and have limited application fields.

Method used

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  • Preparation method of multilayer assembled micron-grade mono-disperse polystyrene magnetic composite microspheres
  • Preparation method of multilayer assembled micron-grade mono-disperse polystyrene magnetic composite microspheres
  • Preparation method of multilayer assembled micron-grade mono-disperse polystyrene magnetic composite microspheres

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Embodiment 1

[0027] This embodiment provides a method for preparing multilayer assembled micron-sized monodisperse polystyrene magnetic composite microspheres, the specific steps are as follows:

[0028] (1) Synthesis of polystyrene microspheres: Take a 250ml round bottom flask, add 3g of polyvinylpyrrolidone (Mw=55000), 100mL of ethanol and 10mL of water, and stir to obtain a homogeneous solution. Weigh 0.08 g of azobisisobutyronitrile as an initiator and dissolve it in 8 mL of distilled styrene monomer. Add the styrene monomer solution dissolved with azobisisobutyronitrile into the homogeneous solution obtained above, stir, protect with nitrogen, raise the reaction temperature to 70°C, and react at 150rpm / min for 20 hours. After the reaction time is up, centrifuge, remove the supernatant, disperse with ethanol, repeat the dispersion six times, and dry in vacuum at 30°C for 24 hours to obtain polystyrene microspheres with a particle size of about 1 μm.

[0029] (2) Sulfonation of polysty...

Embodiment 2

[0035] This embodiment provides a method for preparing multilayer assembled micron-sized monodisperse polystyrene magnetic composite microspheres, the specific steps are as follows:

[0036] (1) Synthesis of polystyrene microspheres: Take a 250ml round bottom flask, add 2.0g polyvinylpyrrolidone (Mw=55000) and 120mL ethanol, stir to obtain a homogeneous solution. Weigh 0.2 g of azobisisobutyronitrile as an initiator and dissolve it in 30 mL of distilled styrene monomer. Add the styrene monomer solution dissolved with azobisisobutyronitrile into the homogeneous solution obtained above, stir, protect with nitrogen, raise the reaction temperature to 68°C, and react at 150rpm / min for 20 hours. After the reaction time is up, centrifuge, remove the supernatant, disperse with ethanol, repeat six times, and vacuum dry at 30° C. for 24 hours to obtain polystyrene microspheres with a particle size of 3 μm.

[0037] (2) Sulfonation of polystyrene microspheres: Take a 100mL round-bottome...

Embodiment 3

[0042] This example provides a method for preparing multilayer assembled micron-sized monodisperse polystyrene magnetic composite microspheres, the specific steps are as follows:

[0043] (1) Synthesis of polystyrene microspheres: Take a 250ml round bottom flask, add 4.0g polyvinylpyrrolidone (Mw=55000), 40mL ethanol and 60mL ethylene glycol methyl ether, and stir to obtain a homogeneous solution. Weigh 0.3 g of azobisisobutyronitrile as an initiator and dissolve it in 30 mL of distilled styrene monomer. Add the styrene monomer solution dissolved with azobisisobutyronitrile into the homogeneous solution obtained above, stir, protect with nitrogen, raise the reaction temperature to 72°C, and react at 150rpm / min for 20 hours. After the reaction time is up, centrifuge, remove the supernatant, disperse with ethanol, repeat six times, and dry in vacuum at 30° C. for 24 hours to obtain polystyrene microspheres with a particle size of 6 μm.

[0044](2) Sulfonation of polystyrene mic...

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Abstract

The invention belongs to the technical field of high molecular functional materials and provides a preparation method of multilayer assembled micron-grade mono-disperse polystyrene magnetic composite microspheres. The preparation method of the polystyrene magnetic composite microspheres comprises the following steps: preparing mono-disperse sulfo or carboxyl polystyrene microspheres with rich negative charges on surfaces by adopting dispersion polymerization; then utilizing electrostatic interaction among the mono-disperse sulfo or carboxyl polystyrene microspheres, strong cation polyelectrolyte and sodium citrate modified Fe3O4 nanoparticles to finally prepare the micron-grade mono-disperse polystyrene magnetic microspheres. According to the preparation method provided by the invention, the mono-disperse polystyrene magnetic microspheres with highly uniform grain diameter and a micron-grade grain diameter can be obtained; the application requirements in the fields of chemiluminescence immunity, cell separation, protein purification and the like can be met; the preparation method provided by the invention also has the advantages of simplicity and convenience for operation, energy saving and consumption reduction and is suitable for industrialized popularization and application.

Description

technical field [0001] The invention belongs to the technical field of polymer functional materials, and in particular relates to a preparation method for multilayer assembly of micron-level monodisperse polystyrene magnetic composite microspheres. Background technique [0002] Magnetic polymer microsphere is a new type of advanced polymer composite functional magnetic material developed in recent years. It is a composite microsphere with certain magnetic properties and special structure formed by combining magnetic inorganic particles and organic polymers by appropriate methods. Magnetic polymer microspheres have both magnetic responsiveness and surface functionality of ordinary polymer microspheres, and are widely used in many fields such as medicine, biochemistry, immunoassay and molecular biology, especially in immobilized enzymes, targeted drugs, etc. It has broad application prospects in cell separation and other aspects. [0003] At present, the methods for preparing...

Claims

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Application Information

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IPC IPC(8): C08L25/06C08K3/22C08K9/04C08J3/12C08F8/00C08F8/36C08F112/08
CPCC08F8/00C08F8/36C08J3/12C08J2325/06C08K3/22C08K9/04C08K2003/2275C08K2201/011C08L25/06C08F112/08
Inventor 程敏卓
Owner 成都爱兴生物科技有限公司
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