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Diquat Raman enhanced spectroscopy for fruits and vegetables and reagent formula thereof

A technology of Raman spectroscopy and enhanced spectroscopy, applied in the field of Raman spectroscopy, can solve the problems of long analysis time, tedious and time-consuming sample pretreatment process, etc., and achieve intuitive and visual analysis results, good linear relationship, simple and fast operation Effect

Inactive Publication Date: 2017-10-10
合肥国研汉因检测科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Gas chromatography / mass spectrometry and liquid chromatography / mass spectrometry are the standard methods recommended by our country, while conventional chromatography requires tedious and time-consuming sample pretreatment process and long analysis time

Method used

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  • Diquat Raman enhanced spectroscopy for fruits and vegetables and reagent formula thereof
  • Diquat Raman enhanced spectroscopy for fruits and vegetables and reagent formula thereof
  • Diquat Raman enhanced spectroscopy for fruits and vegetables and reagent formula thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1 A Raman-enhanced spectroscopy method for diquat in fruits and vegetables and its reagent formulation, comprising the following specific steps:

[0031] 1. Sample preparation: Beat the fruit into a pulp and weigh 3.0g, add 3mL of deionized water to mix, centrifuge at 8000rpm for 10min at room temperature, pass through a 0.22μm filter membrane for testing.

[0032] 2. Sampling: Take 100 μL of reagent A and add it to the detection pool; take 100 μL of NaCl aqueous solution and add it to the detection pool with reagent A added in advance; take 300 μL of the filtrate and add it to the detection pool with reagent A and NaCl in advance, to be tested.

[0033]3. Detection: The portable Raman spectrometer developed by the Chinese Academy of Inspection and Quarantine is used for detection. The emission wavelength of the laser light source is 785nm, the laser energy parameter is selected as 300mw, and the integration time is 10s to adjust the optimal Raman peak intensi...

Embodiment 2

[0034] Embodiment 2 A Raman-enhanced spectroscopy method for diquat in fruits and vegetables and its reagent formulation, comprising the following specific steps:

[0035] 1. Sample preparation: beat the vegetables into a pulp and weigh 3.0g, add 3mL of deionized water to mix, centrifuge at 10000rpm at room temperature for 15min, pass through a 0.22μm filter membrane for testing.

[0036] 2. Sampling: Take 100 μL of reagent A and add it to the detection pool; take 100 μL of NaCl aqueous solution and add it to the detection pool with reagent A added in advance; take 300 μL of filtrate and add it to the detection pool with reagent A and NaCl in advance, to be tested .

[0037] 4. Detection: The portable Raman spectrometer developed by the Chinese Academy of Inspection and Quarantine is used for detection. The laser energy parameter is selected as 300mw, and the optimal Raman peak intensity is adjusted. Use data processing software with background subtraction function for data p...

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Abstract

The invention provides a diquat Raman enhanced spectroscopy for fruits and vegetables and a reagent formula thereof. The method is characterized in that the Raman spectrum signal of diquat is enhanced by adopting a silver colloid aqueous solution A as a surface enhancing reagent and an aqueous solution B containing a certain concentration of Cl- as an auxiliary reagent, and a diquat test is carried out with the characteristic Raman spectrum signal near the wave number 1593cm<-1>. Fruits and vegetables are uniformly mixed, centrifuged and directly tested by using deionized water as an extraction reagent. The method can be used for qualitatively detecting and initially screening the diquat in a food sample and quantitatively detecting the diquat in the food sample by adopting an external standard method according to the linear relationship between the Raman spectrum signal strength of diquat and content thereof, is simple, convenient and quick to operate, is low in cost for reagents and consumable materials, and is easy to popularize and generalize.

Description

technical field [0001] The invention relates to a rapid detection technology for pesticide residues, in particular to Raman spectroscopy, a reagent formula and a use method for on-site rapid detection of diquat in fruits and vegetables. Background technique [0002] Food safety is a "popular project" about the national economy and the people's livelihood, which is directly related to the health and life safety of the broad masses of the people. Moreover, food safety incidents affect a wide range of areas, especially after the application of pesticides in agricultural production, some pesticides remain directly or indirectly in grains, vegetables, fruits, livestock products, aquatic products, and soil and water, which poses a serious threat to human health and safety and even the living environment. growing threat. Therefore, the safe, fast and effective detection of pesticide residues in agricultural products such as fruits and vegetables has become a common concern of scie...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 邹明强齐小花卢春梅王纪平吴渺渺
Owner 合肥国研汉因检测科技有限公司
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