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High-efficiency culture system for large-scale cell production and culture and application method

A cell culture and culture system technology, applied in the field of cell industrial production, can solve the problems of patient treatment hazards, delayed treatment plans, inability to meet, etc., and achieve the effects of reducing pollution risks, simplifying cell culture steps, and reducing difficulty.

Pending Publication Date: 2017-10-10
国健亦诺生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, maintaining a sterile environment during the cell culture process takes up most of the time in the whole process, and the manpower, material and financial resources consumed are immeasurable. It will lead to pollution, which will eventually lead to the delay of a series of treatment plans and cause harm to the treatment of patients
Large-scale, high-demand cultured cells are the key to cell therapy. Relying solely on the cultivation of specialized personnel in a laboratory environment has been unable to meet the growing needs of scientific research, biopharmaceuticals, regenerative medicine, clinical applications, and genetic engineering, and there are still many shortcomings.

Method used

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  • High-efficiency culture system for large-scale cell production and culture and application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Select a highly air-permeable plastic material suitable for cell culture, according to figure 1 prepared as a cell culture bag, and welded the connecting tube to the lower end of the cell culture bag by means of high-frequency heat sealing, wherein the second connecting tube 2 is used for the output of cells for collection; the first connecting tube 1 is used for infusion culture medium; the third connecting tube 3 is used for the input of cells.

Embodiment 2

[0079] Choose high-barrier, air-tight plastic material, according to figure 2 prepared as a cell culture medium preservation bag, and welded the connection tube to the lower end of the cell culture medium preservation bag by means of high-frequency heat sealing, wherein the fourth connection tube 4 and the fifth connection tube 5 are used to transport the cell culture medium to the cell culture bag. Inject the cell culture medium; there is a scale on the cell culture medium storage bag.

Embodiment 3

[0081] In an open environment, use a sterile heat-sealing adapter to heat-seal the first connecting pipe 1 of the cell culture bag to the fourth connecting pipe 4 or the fifth connecting pipe 5 of the cell culture medium storage bag, and then culture fresh cells. The base is injected into the cell culture bag for cell expansion and culture.

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Abstract

The invention relates to a high-efficiency culture system for large-scale cell production and culture and an application method. The culture system comprises at least one cell culture bag and at least one cell culture medium storage bag, the cell culture bags are used for culturing cells, the cell culture medium storage bags are used for storing prepared cell culture media and infusing the cell culture media for cell culture of the cell culture bags, the cell culture bags and the cell culture medium storage bags have different specifications, bag bodies are independent at ordinary times, and heat-seal connection and disconnection under an aseptic condition are ensured by an aseptic heat-seal tube sealing machine when cell culture medium infusion is needed. The probability of cell pollution and infection of operators is reduced, operation safety is improved, and cell culture work efficiency is increased.

Description

technical field [0001] The invention relates to the technical field of cell industrial production, in particular to a high-efficiency culture system and application method for large-scale cell production and culture. Background technique [0002] Cell culture is a process of cultivating cells in an artificial environment, using nutrients, etc., to multiply and proliferate, and to use them for various purposes. Cell culture has been widely used in various fields such as scientific research, biopharmaceuticals, regenerative medicine, clinical application and genetic engineering. [0003] With the advancement of society and the vigorous development of science and technology, human beings have higher expectations for the quality of life and life expectancy. It is everyone's dream to have a healthy, happy and happy life cycle. But like cancer and other chronic diseases, the side effects of traditional treatment methods also make many patients miserable. [0004] Cell therapy i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/02C12M3/04
CPCC12M23/14C12M29/00
Inventor 顾雨春刘建强
Owner 国健亦诺生物科技(北京)有限公司
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