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Preparation method for decellularized tissue membrane

A decellularized tissue and tissue technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of high cost, high price, and long processing time, and achieve short processing cycle, small tissue structure damage, and cost reduction Effect

Active Publication Date: 2017-10-10
IMEIK TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The operation method of decellularized tissue disclosed in Chinese patent CN 103191466 B is simple, but the time required for the tissue preservation step is as long as 1 to 12 months, which makes the whole processing process too long
CN103432627 B adopts steps such as alkali bubble, ribonuclease digestion, alpha-galactosidase, virus inactivation, sterilization, etc., can well remove cell component and alpha-Gal antigen, but wherein ribonuclease and alpha-galactosidase Enzyme preparations such as lactosidase are expensive and costly to process

Method used

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  • Preparation method for decellularized tissue membrane
  • Preparation method for decellularized tissue membrane
  • Preparation method for decellularized tissue membrane

Examples

Experimental program
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Effect test

Embodiment 1

[0032] A method for preparing acellular tissue membrane:

[0033] (1) Take out the peritoneal membrane of 6-month-old cattle and wash them;

[0034] (2) Tissue pruning: Using the peritoneal membrane as the raw material, use scissors to cut off residual tissue fragments such as fat tissue, and rinse off blood stains with purified water.

[0035] (3) Soaking in lye: Weigh 10g of the membrane tissue after the above cleaning, add 100mL of 0.1mol / L sodium hydroxide solution and soak for 20min; take the membrane tissue after soaking in the above steps and add 4% mass concentration Soak the membrane in acetic acid solution for 10 minutes, and then take the membrane into purified water and wash it 3 times, 5 minutes each time.

[0036] (4) Surfactant solution immersion: The formulation of the surfactant solution is 1% sodium lauryl sulfate, 50mM sodium acetate, 20mM potassium chloride, 10mM magnesium chloride, 10mM sodium chloride, 5mM β-mercaptoethanol. Adjust the pH value to 7.0 with aceti...

Embodiment 2

[0042] (1) Take out the membrane: Take out the peritoneal membrane of the 6-month-old cow and wash it;

[0043] (2) Tissue pruning: Using the peritoneal membrane as raw material, use scissors to cut off residual tissue fragments such as fat tissue, and rinse off blood stains with purified water.

[0044] (3) Soaking in lye solution: Weigh 10g of the membrane tissue after the above cleaning, add 100mL of sodium hydroxide solution with a concentration of 1mol / L and soak for 1h; take the membrane tissue after immersion in the above steps and add 4% acetic acid. Soak in the solution for 10 minutes, then take the membrane into purified water and wash it 3 times, 5 minutes each time.

[0045] (4) Surfactant solution immersion: The formulation of the surfactant solution is 1% sodium lauryl sulfate, 50mM sodium acetate, 20mM potassium chloride, 10mM magnesium chloride, 10mM sodium chloride, 5mM β-mercaptoethanol. Adjust the pH value to 7.0 with acetic acid; put the membrane from the previou...

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Abstract

The invention relates to a preparation method for a decellularized tissue membrane. The method comprises the following steps: 1) removing adipose tissue on the tissue membrane, washing and cleaning bloodstain; 2) placing the neutralized and washed tissue in surfactant solution and soaking, and adequately removing impure proteins; 3) placing in protein renaturation solution and soaking; 4) placing in an organic solvent and soaking, and separating out grease; 5) placing in alkaline solution and soaking, neutralizing, and preparing pores on the tissue membrane; 6) placing the tissue membrane in solution containing a crosslinking agent and soaking, crosslinking; and 7) executing the radiation sterilization to the tissue membrane obtained in the steps in the wet state; or executing the radiation sterilization or EO sterilization in the freeze-drying mode. The method is capable of removing antigens, such as cells, lipids and impure proteins, in the human source or animal source tissue membrane, keeping and extracellular matrix and generating the suitable pores. The prepared decellularized tissue membrane can be used as a tissue engineering bent, a patching material for tissue membrane damage and a wound surface dressing.

Description

Technical field [0001] The invention relates to a preparation method of decellularized tissue membrane, which is a method for processing mammalian tissue membrane as a raw material into a medical material. The material can be used for the preparation of tissue engineering scaffolds, tissue membrane repair materials and medical dressings. Background technique [0002] Acellular tissue membrane materials are widely used as medical devices in the medical field, such as biological patches, wound dressings, wound repair materials, tissue repair scaffolds, etc. There are a wide range of raw materials for the preparation of decellularized tissues, such as human skin, animal skin, peritoneal membrane, body cavity membrane, tendon, small intestinal mucosa, amniotic membrane, blood vessels, pericardium, etc. The general method of preparing acellular tissue membrane is to remove the cellular components of the tissue (such as DNA, protein, polysaccharide) from the fresh tissue through physic...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50A61L27/56A61L15/40
CPCA61L15/40A61L27/3633A61L27/3687A61L27/3691A61L27/50A61L27/56A61L2430/40
Inventor 简军陈雄伟刘玉保佘超民张君
Owner IMEIK TECH DEV CO LTD
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