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A kind of antibacterial gel and preparation method thereof

A gel and gellan gum technology, which is applied in pharmaceutical formulations, cosmetic preparations, cosmetic preparations, etc., can solve the problems of no public reports of gellan gum grafted with nisin, and improve the utilization rate of carboxyl groups , enhanced stability, and easy-to-gel effects

Active Publication Date: 2019-09-03
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The theoretical basis and related experiments can show that gellan gum can be used as an excellent protein carrier, but before this application, there is no public report or research on the use of gellan gum for grafting nisin

Method used

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  • A kind of antibacterial gel and preparation method thereof
  • A kind of antibacterial gel and preparation method thereof
  • A kind of antibacterial gel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Preparation of antibacterial washing gel

[0040] (1) Weighing: 100 parts of low acyl gellan gum, 500 parts of nisin, 1000 parts of 2-(N-morpholine)ethanesulfonic acid, 1-(3-dimethylaminopropyl)-3-ethyl 30 parts of carbodiimide hydrochloride, 30 parts of N-hydroxysulfosuccinimide, and 100 parts by weight of deionized water are weighed for later use.

[0041] (2) Gellan gum pretreatment:

[0042] Deacylization: Gellan gum needs to undergo hot alkali treatment. The treatment method is to dissolve gellan gum solid powder with 0.05M sodium hydroxide solution, prepare a solution with a final concentration of 1% (w / v), boil it for 10 minutes, and the solution will be golden yellow. Cool to room temperature and dialyze with deionized water until the dialysate is neutral and the yellow color fades to colorless, clear and transparent.

[0043] Deionization: Add the cation exchange resin to the low acyl gellan gum solution at a concentration of 30% (w / v), and stir for 30 minu...

Embodiment 2

[0050] Example 2: Preparation of antibacterial washing gel

[0051] (1) Weighing: 90 parts of low acyl gellan gum, 450 parts of nisin, 1100 parts of 2-(N-morpholine)ethanesulfonic acid, 1-(3-dimethylaminopropyl)-3-ethyl 20 parts by weight of carbodiimide hydrochloride, 20 parts by N-hydroxysulfosuccinimide, and 90 parts by weight of deionized water are weighed for later use.

[0052] (2) Gellan gum pretreatment:

[0053] Deacylization: Gellan gum needs to be treated with hot alkali. The treatment method is to dissolve the solid powder of gellan gum with 0.05M sodium hydroxide solution, prepare a solution with a final concentration of 1% (w / v), boil for 10 minutes, and the solution will be golden yellow. Cool to room temperature and dialyze with deionized water until the dialysate is neutral and the yellow color fades to colorless, clear and transparent.

[0054] Deionization: Add the cation exchange resin to the low acyl gellan gum solution at a concentration of 10% (w / v), and stir f...

Embodiment 3

[0061] Example 3: Preparation of antibacterial washing gel

[0062] (1) Weighing: 110 parts of low acyl gellan gum, 450 parts of nisin, 900 parts of 2-(N-morpholine)ethanesulfonic acid, 1-(3-dimethylaminopropyl)-3-ethyl 40 parts by weight of carbodiimide hydrochloride, 40 parts by N-hydroxysulfosuccinimide, and 110 parts by weight of deionized water are weighed for later use.

[0063] (2) Gellan gum pretreatment:

[0064] Deacylization: Gellan gum needs to be treated with hot alkali. The treatment method is to dissolve the solid powder of gellan gum with 0.05M sodium hydroxide solution, prepare a solution with a final concentration of 2% (w / v), boil it for 20 minutes, and the solution will be golden yellow. Cool to room temperature and dialyze with deionized water until the dialysate is neutral and the yellow color fades to colorless, clear and transparent.

[0065] Deionization: Add the cation exchange resin to the low acyl gellan gum solution at a concentration of 50% (w / v), and st...

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Abstract

The invention discloses antibacterial gel and a preparation method thereof, and belongs to the field of gel preparation. The antibacterial gel comprises deacylation gellan gum, nisin, 2-(N-morpholine) ethanesulfonic acid, 1-(3-dimethylamino propyl)-3-ethyl carbodi-imide hydrochloride, N-hydroxy succinimide thiosulfate and deionized water. The preparation method includes the steps: resin pretreatment; solution preparation; activation; acylation; dialysis; gel forming. The deacylation gellan gum is covalently grafted to the nisin to form the antibacterial gel, so that the antibacterial gel has the advantages the antibacterial gel is high in grafting ratio, low in cost, easy to form, easily formed, thermally reversible, free from toxicity and the like, and the antibacterial gel is suitable for preparation of antibacterial washing gel.

Description

Technical field [0001] The invention relates to an antibacterial gel and a preparation method thereof, and belongs to the field of gel preparation. Background technique [0002] Nisin has a very good inhibitory effect on spore-producing Gram-positive bacteria, and can be degraded and digested by enzymes in the human body. It is a safe, non-toxic and natural new food preservative. Although nisin has high antibacterial properties, its antibacterial activity and solubility are the main reasons that limit its application. Its activity and stability are significantly reduced by the increase of pH. Nisin is at pH=2~5 Stable, the activity loss is within 20%; pH=6-7, and the potency loss is more than 35%. Nisin was dissolved in a hydrochloric acid solution with pH=3 at 121°C for 15min and still maintained 100% activity after autoclaving for 15min; while pH=6 or 7, activity was completely lost after autoclaving at 121°C for 20min. Therefore, this property severely limits the application...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/64A61K8/73A61K8/04A61Q19/10A61Q17/00C08B37/00C08J3/075C08L5/00
Inventor 詹晓北王支海高敏杰朱莉
Owner JIANGNAN UNIV
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