Rice ARP (Auxin Response Protein) gene cloning and application

An auxin and gene technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of lack of, restricting the high-level development of molecular breeding and molecular design breeding, etc. Effect

Pending Publication Date: 2017-10-03
TIANJIN AGRICULTURE COLLEGE
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  • Summary
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the serious lack of important functional genes with independent intellectual property rights is a "bottleneck" that seriously restricts the high-level development of molecular breeding and molecular design breeding in my country

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0011] The implementation of the present invention will be described in further detail below. The following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention.

[0012] A rice auxin response protein gene ARP, the gene is located on chromosome 4 of the rice genome, 3.8 kb away from the 4,5,7-trihydroxyflavanone dioxygenase gene, the gene has no intron, and the size is 462 base sequence, its gene sequence is SQ1.

[0013] An application of the rice auxin response protein gene ARP, the application is to apply the rice auxin response protein gene ARP to the fields of rice transgenic breeding, molecular design breeding and rice dwarf breeding.

[0014] The discovery and verification process of the rice auxin-responsive protein gene ARP described above includes the following steps:

[0015] (1) Preparation of rice transformed receptors

[0016] The mature seeds of rice variety Nipponbare are shelled, soaked in 70% alcohol...

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Abstract

The invention relates to rice ARP (Auxin Response Protein) gene cloning and application. A rice ARP gene is located on a 4th chromosome of a rice genome and is spaced from a 4,5,7-trihydroxy flavanone dioxygenase gene by a 3.8-kb position; the rice ARP gene has no intron, and a gene sequence of the rice ARP gene is SQ1. The application of the rice ARP gene cloning is characterized by applying the rice ARP gene to the fields of rice transgenic breeding, molecular design breeding and rice dwarf breeding. The rice ARP gene disclosed by the invention can be widely applied to the field of molecular breeding of crops, promotion on plant height reducing can be brought for molecular breeding and dwarf breeding of the crops of the rice and the like, great influence on breeding of the crops of the rice and the like can be generated, the requirement conditions on lab and field fertilization management during an implementation and popularization process are loose, the plant height dwarf effect is remarkable, and a set of effective methods is provided for gene cloning and molecular breeding.

Description

technical field [0001] The invention belongs to the technical field of application of rice functional genomics, in particular to the cloning and application of rice auxin response protein gene ARP. Background technique [0002] Rice is one of the three major food crops. More than one-third of the world's population uses it as the main source of food. It plays a pivotal role in the national economy. With the continuous increase of the world population and the increase of the demand for food, the annual demand for rice is constantly increasing. Today, with the shrinking land area, how to make rice production stable and high to alleviate the impact of the increase in population on rice production The pressure brought by it is a difficult problem faced by the world, and it is also a major scientific research topic in front of scientific researchers. Although traditional genetic research and conventional breeding have made important contributions to the improvement of rice yield...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8261
Inventor 裴忠有宋东颖赵飞陈晓木罗峰孙守钧段霞飞吕建澎王玲
Owner TIANJIN AGRICULTURE COLLEGE
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