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Preparation method and application of amphiphilic chitosan derivative protein adsorption-resistant coating

A technology of chitosan derivatives and proteins, which is applied in the fields of biomedical materials and polymer chemistry, can solve problems such as difficult fixation of solid surfaces, reduced mechanical strength, and difficulty in copolymerization, so as to improve amphiphilic properties, improve membrane properties, The effect of good application prospects

Inactive Publication Date: 2017-10-03
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(2) Anti-protein adsorption materials based on zwitterionic polymers. Now, the research on materials containing zwitterionic polymers is still in the initial stage. Due to the high polarity of zwitterionic polymers, it can only be dissolved in highly polar solvents For example, in water or ethanol, it is very difficult to copolymerize with other low-polarity monomers; on the other hand, the swelling of materials containing zwitterionic polymers in water causes a decrease in their mechanical strength and is not easy to fix on solid surfaces. Performance could be improved
Amphiphilic chitosan is obtained by protecting the amino groups of chitosan and connecting long hydrophobic chains to the hydroxyl groups of chitosan. The literature on the use of the obtained amphiphilic chitosan in anti-protein research has not yet been reported.

Method used

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  • Preparation method and application of amphiphilic chitosan derivative protein adsorption-resistant coating
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  • Preparation method and application of amphiphilic chitosan derivative protein adsorption-resistant coating

Examples

Experimental program
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Effect test

Embodiment 1

[0054] (1) Activation of cholesterol chloroformate

[0055] In a 250 mL round bottom flask, add 1.38 g cholesterol chloroformate (Chol-Cl), 100 mL sequentially N,N - Dimethylformamide (DMF) and 0.60 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), the reaction was stirred at 45 °C for 2 h to obtain no Color clear solution A;

[0056] (2) Synthesis of anti-protein amphiphilic cholesterol chitosan ester (CHCS)

[0057]In a 250 mL three-neck flask, add 1.0 g of chitosan, followed by 35 mL of methanesulfonic acid, seal it with nitrogen gas to remove oxygen, and stir until the chitosan is completely dissolved. The solution A in the reaction process continued to precipitate out, and the reaction was stirred at 45°C for 72 h. After the reaction, the above mixture is poured into a large amount of ice water, the product is washed after centrifugation, and then the obtained washed product is purified by chloroform dissolution-methanol precipitation, then suctio...

Embodiment 2

[0059] (1) Activation of cholesterol chloroformate

[0060] In a 250 mL round bottom flask, add 2.76 g cholesterol chloroformate (Chol-Cl), 100 mL N,N -Dimethylformamide (DMF) and 1.20 g 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), the reaction was stirred at 45 ° C for 2 h to obtain Color clear solution A;

[0061] (2) Synthesis of anti-protein amphiphilic cholesterol chitosan ester (CHCS)

[0062] In a 250 mL three-necked flask, add 1.0 g of chitosan, followed by 35 mL of methanesulfonic acid, seal it with nitrogen gas to remove oxygen, and stir until the chitosan is completely dissolved. The solution A in the reaction process continued to precipitate out, and the reaction was stirred at 45°C for 72 h. After the reaction, the above mixture is poured into a large amount of ice water, the product is washed after centrifugation, and then the obtained washed product is purified by chloroform dissolution-methanol precipitation, then suction filtered, and ...

Embodiment 3

[0064] (1) Activation of cholesterol chloroformate

[0065] In a 250 mL round bottom flask, add 5.49 g cholesterol chloroformate (Chol-Cl), 100 mL N,N -Dimethylformamide (DMF) and 2.40 g 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), the reaction was stirred at 45 ° C for 2 h to obtain Color clear solution A;

[0066] (2) Synthesis of anti-protein amphiphilic cholesterol chitosan ester (CHCS)

[0067] In a 250 mL three-necked flask, add 1.0 g of chitosan, followed by 35 mL of methanesulfonic acid, seal it with nitrogen gas to remove oxygen, and stir until the chitosan is completely dissolved. The solution A in the reaction process continued to precipitate out, and the reaction was stirred at 45°C for 72 h. After the reaction, the above mixture is poured into a large amount of ice water, the product is washed after centrifugation, and then the obtained washed product is purified by chloroform dissolution-methanol precipitation, then suction filtered, and ...

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Abstract

The invention discloses a preparation method and application of an amphiphilic chitosan derivative protein adsorption-resistant coating. The preparation method comprises carrying out hydrophobic modification on chitosan through cholesteryl chloroformate to obtain an amphiphilic cholesterol-modified chitosan (CHCS), uniformly spin-coating a glass slide with the CHCS solution, carrying out drying at 20-80 DEG C to obtain a CHCS protein adsorption-resistant coating, characterizing physical and chemical properties of a CHCS film and a CHCS monomolecular film, and researching protein adsorption-resistant performances of a series of CHCS coatings. The research result shows that a substrate coated with the CSCH film has good protein adsorption resistance. The preparation method of the protein adsorption-resistant coating has simple processes and utilizes easily controlled conditions. Chitosan has good biocompatibility, blood compatibility, safety, microbial biodegradability, film-forming performances and a wide resource range, can reduce a production cost and is expected to become a novel environmentally friendly anti-fouling material.

Description

technical field [0001] The invention belongs to the field of biomedical materials and polymer chemistry, and particularly relates to a preparation method and application of an amphiphilic chitosan derivative anti-protein adsorption coating. Background technique [0002] Protein is an amphiphilic macromolecular substance, which is an indispensable substance in life and has extremely rich biological functions. However, due to the affinity of the protein surface, it is easy to adsorb on the surface of the material and cause contamination of the material, which in turn affects the performance of the material and produces a series of hazards. In terms of abiotics, marine biosorption is essentially a problem of protein adsorption. Marine organisms such as barnacles, mussels, diatoms and other secretions contain a large amount of proteins and polysaccharides, and their secretions are easily adsorbed on the surface of the material. Disperse, so that fouling marine organisms adsorb ...

Claims

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Application Information

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IPC IPC(8): C09D105/08C09D5/16C08B37/08
CPCC08B37/003C09D5/1662C09D105/08
Inventor 关怀民刘玉洁童跃进
Owner FUJIAN NORMAL UNIV
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