Foot-and-mouth disease marker vaccine strain with 3B protein dominant epitope deficiency and construction method and application thereof

Abstract

The invention provides a foot-and-mouth disease marker vaccine strain with 3B protein dominant epitope deficiency and a construction method and application thereof; 3B1 and 3B2 non-structural protein encoded amino acid sequences of the marker vaccine strain are shown as in SEQ ID No. 8. The vaccine strain constructed herein has mutation modifications (4AGP6-4TAA6) of 3B1 and 3B2 non-structural protein amino acids, wherein the mutations of the 3B1 and 3B2 protein amino acids enable a recombinant virus to be completely deprived of the ability to bind with monoclonal antibody 3B4B1 recognizing 3B1and 3B2 dominant epitopes, and the marked virus has the similar replicating ability as parent viruses. Therefore, the foot-and-mouth disease marker vaccine strain constructed herein is suitable for developing foot-and-mouth disease marker vaccines for distinguishing natural infections and vaccine immunity, and reliable technical support is provided for the construction of control, purification and infection-free zones in China.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a foot-and-mouth disease marker vaccine strain lacking the dominant epitope of 3B protein and its construction method and application. Background technique [0002] Foot-and-Mouth Disease (FMD) is an acute, febrile, highly contagious infectious disease infecting pigs, cattle, sheep and other major domestic animals and wild artiodactyls. The disease spreads rapidly, has a high incidence rate, and is extremely harmful. Therefore, the International Office of Epizootics (OIE) lists it as the first of the must-reported diseases, and my country stipulates it as a first-class animal infectious disease. The outbreak and prevalence of this disease have reduced the production capacity of livestock, stagnated the trade of live animals and animal products, and caused huge economic losses; at the same time, it has caused serious negative effects on public health and na...

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Problems solved by technology

However, the G-H loop of the foot-and-mouth disease virus structural protein VP1 is the main immune gene that induces the body to produce neutralizing antibodies, and the deletion will affect the immunogenicity of the vaccine; there is a risk of virulence reversion to the attenuated vaccine, and the deletion of non-structural protein genes will affect the virus. Therefore, this field still needs to innovate and develop better candidate strains of foot-and-mouth disease marker virus vaccines to provide effective technical support for the effective prevention, control and purification of foot-and-mouth disease and the construction of disease-free areas in my country

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