Monoclonal antibody for anti-HPV (Human Papillomavirus) 16 type E7 protein, test paper and detection kit

Abstract

A monoclonal antibody against HPV16 type E7 protein, a detection test paper and a detection kit. The binding site of monoclonal antibody against HPV16 E7 protein is: amino acid 30-36 of HPV16 E7 protein, amino acid 36-43 of HPV16 E7 protein, amino acid 62-73 of HPV16 E7 protein or HPV16 Amino acids 73-77 of type E7 protein. The detection test paper includes a backing and a sample pad fixed on the backing, a binding pad, an NC film and an absorbent pad; the binding pad is attached and fixed under the sample pad, one end of the NC film is attached and fixed under the binding pad, and the other end Link to each other with water-absorbing pad; NC membrane is sprayed with detection antibody, and detection antibody comprises the monoclonal antibody of the anti-HPV16 type E7 protein of claim 1 and goat anti-mouse secondary antibody, the monoclonal antibody of anti-HPV16 type E7 protein is used as detection line, Goat anti-mouse secondary antibody is used as a quality control line; the binding pad is coated with fluorescent microsphere-labeled antibody. The application can detect HPV type E7 protein simply and quickly.

Description

technical field [0001] The application relates to the field of antibodies, in particular to a monoclonal antibody against HPV16 type E7 protein, a detection test paper and a detection kit. Background technique [0002] Cervical cancer is a cancer with a very high incidence in women and is associated with high-risk human papillomavirus infection in 99.7% of cases. There are about 520,000 cases of cervical cancer and nearly 260,000 deaths worldwide each year. There are more than 100 different isolates of HPV that have been broadly subdivided into high-risk and low-risk subtypes based on their association with cervical cancer or with benign cervical lesions or atypical hyperplasia. Low-risk HPVs include HPV6, 11, 42, 43, 44, etc., which often cause external genital warts and other benign lesions, including low-grade cervical intraepithelial lesions (CIN I), and high-risk HPVs include HPV16, 18, 31, 33, 35 , 39, 45, 51, 52, 56, 58, 59, 68, CP8304 and other subtypes, which are r...

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