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Surface functionalization modification method for metal organic framework (MOF) material based on liposome membrane

A metal-organic framework and surface functionalization technology, which is applied in the field of surface functionalization modification of metal-organic framework materials, can solve the problems of early release of MOFs materials that cannot be improved, poor biological stability, etc., and achieves improved biological stability and biological safety. Simple operation, high biosafety effect

Active Publication Date: 2017-09-22
XIDIAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] To sum up, the problems existing in the existing technology are: the traditional MOFs material surface functional modification method has poor biological stability when applied in the biomedical field, and cannot improve the early release of MOFs materials when delivering imaging molecules or therapeutic drugs.

Method used

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  • Surface functionalization modification method for metal organic framework (MOF) material based on liposome membrane
  • Surface functionalization modification method for metal organic framework (MOF) material based on liposome membrane
  • Surface functionalization modification method for metal organic framework (MOF) material based on liposome membrane

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preparation example Construction

[0057] The synthetic method of MOFs material of the present invention specifically comprises:

[0058] (1) Synthesize UiO series and ZIF series by solvothermal synthesis. The specific steps are to weigh an appropriate amount of metal ions and organic ligands, ultrasonically disperse them in the corresponding solvent, add an appropriate amount of regulator, and transfer them to a hydrothermal kettle , react at a certain temperature for a period of time, centrifuge and ultrasonically redisperse at a certain speed, and wash with a suitable solvent several times to obtain the final product.

[0059] When UiO-66 is synthesized in the above steps, the metal ion is anhydrous zirconium chloride, and the amount weighed is 10-30 mg, specifically 25 mg; the organic ligand is terephthalic acid, and the amount weighed is 30-80 mg, specifically It can be 40mg; the reaction solvent is N,N-dimethylformamide, the measured amount is 3-6mL, specifically 4mL; the regulator is glacial acetic acid,...

Embodiment 1

[0076] Preparation of liposomes

[0077] Preparation of DOPA liposomes

[0078] Take by weighing 5mg lipid, wherein the mass ratio of various lipid components is DOPA / chol / 18:1PEG-2000PE=15:6:1, be dissolved in the mixed solution of 2mL chloroform and methanol (volume ratio is 4:1 ), dissolved and mixed evenly, the organic solvent was sucked dry with a rotary evaporator under the condition of a water bath at 35°C, and dried under vacuum at room temperature overnight. Add 4ml of HEPES buffer (100mMNaCl, 10Mm HEPES, pH 7.4) to hydrate at room temperature for 1-2h, and then extrude 21 times with a liposome extruder to obtain DOPA liposomes with a size of 120-130nm. like figure 2 (a) shows the hydrodynamic diameter diagram of DOPA liposome, such as figure 2 (b) shows the potential diagram of DOPA liposomes.

[0079] Preparation of DOPC liposomes

[0080] Take by weighing 5mg lipid, wherein the mass ratio of various lipid components is DOPC / chol / 18:1PEG-2000PE=15:6:1, be dis...

Embodiment 2

[0084] Preparation of MOFs materials

[0085] Preparation of UiO-66

[0086] Weigh 15.5mg zirconium chloride (ZrCl 4 ), 50mg terephthalic acid (H 2 BDC), dissolved in 4ml of N,N-dimethylformamide (DMF), dissolved completely, added 0.3-0.9ml of acetic acid, transferred to a hydrothermal reaction kettle, reacted at 90-100°C for 18h, and the obtained solution Centrifuge at 10000rpm for 10min, and wash twice with ethanol and water respectively by ultrasonic dispersion and centrifugation to obtain UiO-66, whose hydrodynamic size is 140-900nm. like image 3 (a) shows the SEM image of UiO-66 with a hydrodynamic diameter of 230 nm, as image 3 (b) shows the TEM image of UiO-66 with a hydrodynamic diameter of 150 nm.

[0087] Preparation of MIL-100

[0088] Weigh 486mg of ferric chloride hexahydrate (FeCl 3 ·6H 2 O), 140 mg of isophthalic acid (H 3 BTC), dissolved in the mixed solution of 5ml water and ethanol, wherein the volume ratio of water and ethanol is 1:0.25~4, complet...

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Abstract

The invention belongs to the technical field of preparation of nanomaterials and discloses a surface functionalization modification method for a metal organic framework (MOF) material based on a liposome membrane. The surface functionalization modification method comprises the following steps: selecting MOFs materials and liposome which are mutually matched, and enabling an electrostatic adsorption or covalent linkage effect to exist between the liposome membrane and exposed active sites of the MOFs materials; directly mixing corresponding MOFs materials and a liposome solution, and carrying out fusion coating and centrifugal washing to obtain a final product; carrying out quality evaluation and performance evaluation on the final product, coating the MOFs materials with the liposome by electrostatic adsorption or covalent linkage, and finishing surface functionalization modification of the MOFs materials. Compared with a traditional polyethylene glycol modification method, the surface functionalization modification method has the advantages that higher biological stability can be provided, simplicity and convenience in operation are realized, and modification efficiency is relatively high; no organic solvents or toxic solvents are needed; the MOFs materials are expected to be endowed with novel functions when the surface functionalization modification method is applied to the aspects of drug delivery, molecular image and the like.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial preparation, and in particular relates to a surface functional modification method of a liposome membrane-based metal-organic framework material. Background technique [0002] Metal-organic frameworks (MOFs) are three-dimensional porous materials formed by coordination with inorganic metal ion clusters as coordination nodes and organic ligands as connecting units. Due to its diverse structural composition, high specific surface area, adjustable pore size, adjustable shape size, and good biocompatibility, MOFs materials are widely used in drug delivery, molecular imaging, biosensing, etc. biomedical field. From the structural characteristics of metal-organic framework materials, there are uncoordinated metal ion binding sites and unconnected organic ligand connection nodes on the surface, so the surface must be functionally modified to improve biomedical applications. Its biostability and bi...

Claims

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Application Information

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IPC IPC(8): C08G83/00
CPCC08G83/008
Inventor 王忠良乔晁强张瑞丽王永东张象涵田捷
Owner XIDIAN UNIV
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