GSTT1 genotyping method based on quantitative PCR technology

Abstract

The invention discloses a GSTT1 genotyping method based on quantitative PCR technology. According to the GSTT1 genotyping method based on quantitative PCR technology, GSTT1 gene specific primers SEQ1 and SEQ2 are used for amplification of GSTT1 gene segments, and GSTT1 gene specific TAQman probe SEQ3-Fam is designed in amplification regions defined by the GSTT1 gene specific primers at the same time; ALbumin specific PCR primers SEQ4 and SEQ5 are used for amplification of ALbumin gene segments, and ALbumin gene specific TAQman probe SEQ6-Vic is designed in amplification regions defined by the ALbumin gene specific primers at the same time. The GSTT1 genotyping method is capable of protecting PCR products from being polluted, avoiding pollution caused by toxic DNA coloring agents, simplifying detection and result analysis process greatly, and increasing detection flux.

Description

technical field [0001] The invention relates to the technical field of gene detection, in particular to a GSTT1 typing detection method based on quantitative PCR technology. Background technique [0002] GSTT1 is an important metabolic enzyme involved in the transformation and metabolism of endogenous and exogenous substances, endowing metabolites with new biological properties. GSTT1 is polymorphic in humans, and GSTT1 deletion is a common genotype. There are differences in the transformation function of the individual geomaterials of different GSTT1 genotypes. GSTT1 genotyping is used in the study of various biological traits. Traditionally, since the GSTT1 deletion polymorphism can be detected by ordinary PCR, conventional gene-specific PCR and agarose electrophoresis detection methods are often used to confirm the GSTT1 genotype. Although it is simple, it is easy to cause laboratory pollution because the electrophoresis operation must be opened to add samples and elec...

AI Technical Summary

Problems solved by technology

Although it is simple, it is easy to cause laboratory pollution because the electrophoresis operation must open the tube to add samples and electrophoresis, and it is difficult to ensure the reliability of the experiment under high throughput

At the same time, the conventional PCR electrophoresis analysis method is also difficult to find the heterozygous genotype, which affects the accurate determination of gene traits analysis.

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