Method for domesticating and culturing activated sludge for degrading penicillin by using glucose as co-substrate

Abstract

The invention discloses a method for domesticating and culturing activated sludge for degrading penicillin by using glucose as a co-substrate. The method comprises the following steps of obtaining the reflux sludge from a secondary settling tank of a domestic sludge treatment plant as seed sludge, using the glucose as the co-substrate of penicillin to provide a carbon source for the system, operating a membrane biological reactor to perform aerobic treatment on the penicillin wastewater, and gradually increasing the load of the reactor; after stably running, continuously increasing the concentration of the penicillin in the intake water by gradients, and reducing the concentration of glucose in the intake water, until the carbon source is completely replaced into the penicillin; in the process, enabling a bacterial flora in the membrane biological reactor to continuously adapt to the stress of the penicillin, so as to form novel microorganism ecology. The domesticating method has the advantages that the troublesome bacteria screening step is not needed, the operation is simple and flexible, and the existing functional microorganism which is difficult in separating and culturing can be furthest utilized; by using the degradable glucose as the co-substrate, the bacterial flora with degrading function can be quickly screened from the penicillin, so as to apply to the high-efficiency treatment of the penicillin wastewater.

Description

technical field [0001] The present invention relates to a method for degrading penicillin activated sludge by using glucose as a co-substrate, and also relates to the application of the above-mentioned method for degrading penicillin activated sludge by using glucose as a co-substrate for degrading penicillin wastewater, which belongs to microbial wastewater treatment technology field. Background technique [0002] Due to the gradual increase in environmental protection thresholds in developed countries, the environmental protection treatment costs of their pharmaceutical companies have continued to soar and their profits have dropped sharply. In order to reduce production costs, many pharmaceutical companies with heavy pollution have largely transferred to developing countries with relatively low environmental protection requirements (China, India, etc.). At present, the domestic antibiotic industry is in a stage of rapid development. The annual output of penicillin exceed...

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Problems solved by technology

For example, the invention patent with the Chinese patent application number 201310148812.6 discloses a preparation method of an antibiotic drug erythromycin with high-efficiency degradation bacterial agent, and the invention patent with the Chinese patent application number 201610109635.4 discloses a screening of fosfomycin-degrading bacteria and its bacterial agent Preparation method and application, the invention patent of Chinese patent application number 201610895774.4 discloses a high-efficiency antibiotic wastewater treatment bacterial agent and its preparation method and application. The above three applications are all separated by dilution coating and plate marking and then processed Cultivation and purification, although antibiotics can be efficiently degraded by bacterial agents, which can speed up the domestication process of antibiotic wastewater treatment activated sludge, but the screening process is cumbersome and the workload is large; the type and composition of functional strains are relatively single, and the actual application adaptability is relatively low. Poor; in addition, more than 90% of microorganisms in the environment are difficult to isolate and culture, this method severely limits the utilization of functional microbial resources

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