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Recombinant delta fHbp-NadA fused protein vector and preparation method and application thereof

A technology of fusion protein and carrier, which is applied in the direction of recombinant DNA technology, fusion polypeptide, antigen-carrier connection, etc., can solve the problems of reducing the immunogenicity of polysaccharides in conjugated vaccines and high total concentration, so as to achieve the best immune effect and reduce the risk Effect

Active Publication Date: 2017-09-12
BRAVOVAX +1
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Problems solved by technology

The reason is that the total concentration of tetanus toxoid and diphtheria toxoid as carriers in the multivalent pneumococcal conjugate vaccine is too high, which will cause the carrier receptor competitive inhibition effect when inoculated at the same time with the combination vaccine containing DPT component. Reduced immunogenicity of the polysaccharide portion of the conjugated vaccine

Method used

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  • Recombinant delta fHbp-NadA fused protein vector and preparation method and application thereof
  • Recombinant delta fHbp-NadA fused protein vector and preparation method and application thereof
  • Recombinant delta fHbp-NadA fused protein vector and preparation method and application thereof

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Embodiment Construction

[0049] A ΔfHbp-NadA protein carrier and a multivalent meningococcal and pneumococcal conjugate vaccine based on the ΔfHbp-NadA protein carrier provided by the present invention will be further described below in conjunction with specific examples. The embodiments described below are exemplary only for explaining the present invention and should not be construed as limiting the present invention.

[0050] The experimental methods in the following examples are conventional methods unless otherwise specified. The experimental materials used in the following examples were purchased from the market unless otherwise specified.

[0051] 1. Cloning and prokaryotic expression of ΔfHbp and ΔfHbp-NadA recombinant proteins

[0052] 1. Construction of Pet-ΔfHbp and Pet-ΔfHbp-NadA recombinant plasmids

[0053] fHbp is a membrane surface lipoprotein, and many strains of meningococcus have its gene, and strains that do not carry the gene have also been found. According to the antigenic cro...

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Abstract

The invention provides a recombinant delta fHbp-NadA fused protein vector and a preparation method and application thereof, and further provides polyvalent meningococcus and pneumococcus conjugated vaccine based on the delta fHbp-NadA fused protein vector. According to the design, two active proteins are subjected to fusion expression through a flexible connecting peptide chain; activities of both the proteins are maintained while the two proteins are fused into the single protein; thus, during industrial production and immunization, controllability is improved, risk is reduced greatly, and more polysaccharide-protein binding sites can be provided.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a recombinant ΔfHbp-NadA protein carrier for multivalent meningococcal and pneumococcal conjugate vaccines, a preparation method and application thereof. Background technique [0002] 1. Pathogenic microorganisms and vaccines [0003] Microorganisms that can cause infectious diseases in humans or animals are called pathogenic microorganisms or pathogenic microorganisms. Infection refers to the process of pathogenic microorganisms invading the body, growing and multiplying in certain parts, and causing a series of pathophysiological processes. When pathogenic microorganisms invade the body, the pathogenic microorganisms interact with the body and change each other's activity and function. Therefore, whether they can cause infectious diseases depends on the pathogenicity or virulence of the pathogenic microorganisms on the one hand, and on the other hand. Depends on the b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C12N15/62A61K39/095A61K47/64A61P31/04
CPCA61K39/09A61K39/095A61K39/116A61K39/385A61K2039/57A61K2039/6031A61K2039/6068A61K2039/62A61K2039/70C07K14/22C07K2319/00C12N15/62C12N15/70A61K2300/00
Inventor 吴克
Owner BRAVOVAX
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