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RGD phage/fibroin composite hemostatic material and preparation method thereof

A hemostatic material and bacteriophage technology, applied in the field of biomedical materials, can solve problems such as lack of mechanical properties and discomfort, and achieve the effect of accelerating hemostasis speed and effect, high biocompatibility, and increasing platelet adhesion.

Inactive Publication Date: 2017-09-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in these related studies, hemostatic factors or hemostatic agents are often small chemical molecules or porous materials with poor mechanical properties, which are not suitable for hemostasis of large wounds.
There has never been a report of compounding RGD phage and silk fibroin aqueous solution to prepare a hemostatic material with excellent mechanical properties, rapid hemostasis, and high porosity for hemostasis of human wounds

Method used

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  • RGD phage/fibroin composite hemostatic material and preparation method thereof
  • RGD phage/fibroin composite hemostatic material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Preparation method of RGD phage / silk fibroin hemostatic material with controllable degradation

[0067] 1) The fibrous silk fibroin obtained after silkworm cocoon degumming is sequentially dissolved, filtered, dialyzed and centrifuged, and concentrated to a silk fibroin aqueous solution with a mass percentage of 100 mg / mL;

[0068] 2) Set the concentration to 10 7 The pfu / mL phage solution is ultrasonically dispersed, mixed with the silk fibroin solution, and stirred evenly to obtain the RGD phage / silk fibroin composite solution;

[0069] 3) Vacuum freeze-drying at -20°C for 24 hours to obtain a three-dimensional porous RGD phage / silk fibroin composite hemostatic material;

[0070] 4) Treatment with 30% alcohol can control the degradation rate of the three-dimensional porous RGD phage / silk fibroin hemostatic material within one day.

Embodiment 2

[0072] The preparation method of the RGD phage / silk fibroin hemostatic material of controllable degradation comprises the following steps:

[0073] 1) The fibrous silk fibroin obtained after silkworm cocoon degumming is successively dissolved, filtered, dialyzed and centrifuged, and then concentrated to a silk fibroin aqueous solution with a mass percentage of 1 mg / mL;

[0074] 2) Set the concentration to 10 14 The pfu / mL phage solution is ultrasonically dispersed, mixed with the silk fibroin solution, and stirred evenly to obtain the RGD phage / silk fibroin composite solution;

[0075] 3) Vacuum freeze-drying at -80°C for 48 hours to obtain a three-dimensional porous RGD phage / silk fibroin composite hemostatic material;

[0076] 4) When treated with 80% alcohol, the degradation rate of the three-dimensional porous RGD bacteriophage / silk fibroin hemostatic material can be basically not degraded (the dissolution rate is greater than 80% after 1 month).

Embodiment 3

[0078] The preparation method of the RGD phage / silk fibroin hemostatic material with controllable degradation adopts the following steps:

[0079] 1) The fibrous silk fibroin obtained after silkworm cocoon degumming is sequentially dissolved, filtered, dialyzed and centrifuged, and then concentrated to a silk fibroin aqueous solution with a mass percentage of 0.1 mg / mL;

[0080] 2) Set the concentration to 10 20 The pfu / mL phage solution is ultrasonically dispersed, mixed with the silk fibroin solution, and stirred evenly to obtain the RGD phage / silk fibroin composite solution;

[0081] 3) Obtain a three-dimensional porous RGD phage / silk fibroin composite hemostatic material by freeze-drying;

[0082] 4) Take 2 mL of fresh rabbit blood, put the prepared RGD phage / silk fibroin composite hemostatic material into the fresh rabbit blood, and the fresh rabbit blood coagulates after 3 minutes.

[0083] Therefore, the process of the present invention is simple, and supramolecular s...

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Abstract

The invention belongs to the field of biomedical materials, and relates to a preparation method of a controllably-degraded RGD phage / fibroin composite quick hemostatic material. The invention provides the controllably-degraded RGD phage / fibroin hemostatic material prepared by using a fibroin-prepared bioabsorbable natural biological material as a supporting part and combining the supporting part with RGD phage, and the RGD phage / fibroin hemostatic material has a significant blood coagulation promoting effect. The RGD phage / fibroin hemostatic material is mainly applied to hemostasis of skin wounds and organs, and is an ideal hemostatic material. The RGD phage / fibroin hemostatic material has the following outstanding characteristics: (1) treated fibroin can attract negatively charged platelets and red blood cells in blood so as to increase platelet adhesion and promote thrombosis; (2) after a certain period of time, RGD phage / fibroin porous hemostatic material can be degraded and reabsorbed by a body, so that a scab is not formed at a wound position; and (3) the RGD phage / fibroin composite quick hemostatic material does not simply depend on the hemostatic ability, and through compounding of the RGD phage, the hemostatic speed of the hemostatic material is increased and the hemostatic effect of the hemostatic material is improved.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and relates to a preparation method of a controllable degradable RGD phage / silk fibroin compound rapid hemostatic material. Background technique [0002] Emergency treatment of temporary accidents and trauma hemostasis during surgery require appropriate hemostasis methods for rapid hemostasis, and local and effective rapid hemostasis of patients is very important, which can effectively reduce casualties. Currently, there are three types of rapid hemostasis materials reported: powder (such as zeolite, collagen powder, etc.), fibrous membrane (such as oxidized regenerated cellulose, etc.), and porous sponge materials (such as gelatin sponge, etc.). However, the hemostatic materials made of these raw materials have different mechanisms of action and use methods. Hemostatic drugs mainly promote blood coagulation and achieve hemostasis by enhancing coagulation factors or inhibiting anticoagulant fa...

Claims

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Application Information

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IPC IPC(8): A61L24/10A61L24/00C12N15/70C12N7/01
CPCA61L24/108A61L24/0015A61L24/0036A61L24/0042A61L2300/252A61L2300/412A61L2300/418A61L2300/604A61L2400/04C12N7/00C12N15/70C12N2795/00052C08L89/00
Inventor 毛传斌帅亚俊杨明英
Owner ZHEJIANG UNIV
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