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Hydrothermal method synthesized photosensitive gold-silver alloy nanocluster adopting micromolecular adenosine monophosphate as protection ligand

A technology of adenosine monophosphate and gold-silver alloy, which is applied in the field of gold-silver alloy nanocluster preparation, can solve problems such as limiting the application of nanoclusters, and achieve unique photosensitivity, simple preparation procedures, and uniform sample particle size

Inactive Publication Date: 2017-09-05
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the existence of a large amount of autofluorescence in organisms, the main emission range includes this blue-green light, which greatly limits the application of this type of nanoclusters in biological systems.

Method used

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  • Hydrothermal method synthesized photosensitive gold-silver alloy nanocluster adopting micromolecular adenosine monophosphate as protection ligand
  • Hydrothermal method synthesized photosensitive gold-silver alloy nanocluster adopting micromolecular adenosine monophosphate as protection ligand
  • Hydrothermal method synthesized photosensitive gold-silver alloy nanocluster adopting micromolecular adenosine monophosphate as protection ligand

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] In the process of synthesizing the gold-silver alloy protected by AMP, because sodium citrate is used as reducing agent and there is a large excess, so this item is not included in the optimization of its synthesis conditions. First optimize the synthesis time, keep the ratio of gold, silver and AMP at 1:1:5, respectively 1, 1 and 5mM, hydrothermal synthesis at 120°C, heating 15, 30, 45, 60, 75, 90, 105 and 120 respectively minute. Such as figure 1 As shown in (3), the optimal synthesis time is 30 minutes. Secondly, optimize the ratio of AMP to metal ions, keep the gold-silver ratio of 1:1 as 1mM; adjust the different concentrations of AMP to 2, 5 and 10mM respectively; hydrothermal synthesis at 120°C for 30 minutes. Such as figure 1As shown in (2), if the ligand concentration is too high (>10mM), the emission peak appears at 480nm, which is not conducive to the formation of gold-silver alloy nanoclusters; then the optimal concentration of AMP is 5 times the equivale...

Embodiment 2

[0028] Take 1 mg of the purified gold-silver alloy nanoclusters in Example 1 and dissolve them in 10 ml of aqueous solution with a concentration of 0.1 mg / mL. Take 300 microliters of the above solution and add 700 microliters of deionized water to test the fluorescence spectrum. The test conditions are: optical path slit 5-5, excitation wavelength 354nm, light source: 150W xenon lamp, scanning range 380-680nm, 1cm×1cm 1mL quartz ratio color dish. Such as figure 2 In the excitation spectrum, the optimal excitation wavelength of alloy nanoclusters is determined to be 354nm. The optimal excitation wavelength is used to excite the nanoclusters, and the optimal emission wavelength is 550nm to emit orange light, and the difference between the two, namely the Stokes shift, is 200nm.

Embodiment 3

[0030] Take 10 microliters of 0.1 mg / mL alloy nanoclusters in Example 2, drop them on the micro-grid ultra-thin copper grid, and after standing still for 10 minutes, use filter paper to blot the surrounding solution. After repeating 2 times, let it dry naturally, and test the transmission electron microscope after 12 hours. Such as image 3 As shown in (1): Au / AgNCs@AMP is highly monodisperse and has clear lattice fringes; its lattice spacing is 0.21 nm, which corresponds to the distance between the 111 lattice planes on the face center of a gold atom cube, This shows that the nanoparticles do contain regular metal nanocrystals. In addition, if image 3 As shown in (2), through the statistical analysis of more than 300 particle sizes, it is finally obtained that their average diameter is about 2.05nm, and the particle size distribution of most particles is between 1.95-2.45nm.

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Abstract

The invention discloses a hydrothermal method synthesized photosensitive gold-silver alloy nanocluster adopting micromolecular adenosine monophosphate as a protection ligand, and belongs to the technical field of preparation of gold-silver alloy nanoclusters. The photosensitive gold-silver alloy nanocluster is prepared through the steps of sequentially adding an adenosine monophosphate solid sample, deionized water, a chloroauric acid aqueous solution, a silver nitrate aqueous solution and a sodium citrate aqueous solution into a reaction kettle, wherein adenosine monophosphate is the protection ligand, and sodium citrate is adopted as a reducing agent and reduces trivalent gold ions into nulvalent and univalent gold; after hydrothermal reaction, slowly cooling a reactant to the room temperature, so as to obtain a gold-silver alloy nanocluster product adopting micromolecular adenosine monophosphate as the protection ligand; and carrying out purification through an acetone precipitation method or dialysis method, so as to obtain a target product. The adenosine monophosphate protected fluorescent gold-silver alloy nanocluster has the advantages that operation is simple, efficient, time-saving, eco-friendly and environmentally-friendly and photosensitivity is achieved, the nanocluster gives out orange light, fluorescent quantum yield is relatively high, and the nanocluster has relatively large application potentials in the aspect of biology.

Description

technical field [0001] The invention belongs to the technical field of gold-silver alloy nano-cluster preparation, and specifically relates to a photosensitive gold-silver alloy nano-cluster synthesized by a hydrothermal method with small molecule adenosine monophosphate as a protective ligand. Background technique [0002] Metal nanoclusters (NCs) are a type of zero-dimensional nanomaterials; they consist of only a few or dozens of metal atoms and have comparable Fermi electron wavelengths (ie, the De Broglie wavelength near the Fermi surface) Therefore, it has received extensive attention in the field of nanomaterials. Because the electrons in metal atoms are confined to molecular size and discrete energy levels, metal nanoclusters have unique optical and electrical properties, such as strong photoluminescence, molecular-like energy gap, and high catalytic performance. In addition, since the number of surface atoms and electronic structure are direct factors affecting the...

Claims

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Application Information

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IPC IPC(8): B22F9/24B22F1/00B82Y30/00B82Y40/00
CPCB82Y30/00B82Y40/00B22F9/24B22F1/102B22F1/054
Inventor 李洪伟刘姣吴玉清
Owner JILIN UNIV
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