Nano-composite biological scaffold with multi-stage controllable through hole structure, and preparation method and application thereof

Abstract

The invention discloses a nano-composite biological scaffold with multi-stage controllable through hole structure, and a preparation method and application thereof. The preparation method is combined with a water-in-oil type Pickering high internal phase emulsion template method and a 3D printing technology, the Pickering emulsion template takes an organic solution containing biodegradable polyester and hydrophobic modified silicon dioxide nanoparticles as an oil phase and deionized water as an aqueous phase, the oil phase and the aqueous phase are mixed and emulsified to form water-in-oil type Pickering emulsion, the emulsion is printed and formed by the 3D printing technology, and the nano-composite biological scaffold with the multi-stage controllable through hole structure is obtained. The preparation method provided by the invention is simple in operation, mild in preparation condition, low in requirement on equipment and suitable for industrialized production, the hole structure of the porous scaffold can be adjusted conveniently and rapidly by changing the preparation condition of the emulsion, the scaffold structure is regulated and controlled by writing different printing programs, and design and implementation of the hole structure are unified.

Description

Technical field [0001] The invention belongs to the field of biomedical materials, and specifically relates to a nano-composite biological scaffold with a multi-level controllable through-hole structure, and a preparation method and application thereof. Background technique [0002] Bone tissue engineering is one of the ways to be widely used and concerned in the repair of damaged and diseased bones. Before being implanted in the body for repair, stem cells are usually seeded on a scaffold with good biocompatibility that has a 3D interconnected pore structure and is self-degradable in the body. In application, since the 3D biological scaffold will act as an artificial extracellular matrix, the prepared 3D biological scaffold should be similar to the natural extracellular matrix. First of all, the raw materials used to prepare the 3D bio-scaffold must have good biocompatibility and be able to degrade in the body at an appropriate speed. Second, the product scaffold must have a c...

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Problems solved by technology

The Pickering high internal phase emulsion template method is simple and easy to operate, but usually the prepared scaffolds only have mesopore and small pore structure (Acs Appl. Mater. Interfaces, 2014, 19: 17166-17175; J. Control. Release 2015, E127- E127), thus not providing sufficient space for cell adhesion

As an emerging technology for preparing biological scaffolds, 3D printing technology can achieve precise control of the pore structure of the scaffold, but the preparation of micron (and below) pore structures puts forward higher requirements for mechanical precision and programming difficulty. Greatly increased the cost of preparation

[0005] However, 3D printing has higher requirements on the viscosity of Pickering emulsion, and most of the Pickering emulsions stabilized by solid particles have low viscosity, such as the Pickering high internal phase emulsion stabilized by hydrophobically modified hydroxyapatite nanoparticles, due to its viscosity Cannot meet the requirements of 3D printing (J.Mech.Behav. Biomed., 2014, 88-99), thus limiting its further development

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