Promoter batch capture method

Abstract

The invention provides a promoter batch capture method. According to the promoter batch capture method, when a plant gene is transcribed, a complex of a 'DNA-protein factor' formed in a promoter area is utilized, an improved chromatin immunoprecipitation technology is applied, and DNA containing an unknown plant gene promoter fragment is sorted out. The promoter batch capture method is a technology for specifically capturing an unknown plant gene promoter fragment by utilizing the property that protein factors of OsTBP2 and OsTFIIB are tightly combined with promotion and transcribe of TATA box short-chain conserved sequence which on the promoter and through the generation of a specific antibody of the two factors and through the combination of the chromatin immunoprecipitation technology (ChIP). In the technology, by utilizing the advantages that most promoters contain one TATA box short-chain conserved sequence, and through the combination of technology that the unknown plant gene promoter fragment is separated through the newly developed chromatin immunoprecipitation technology (ChIP), large-scale capture of the plant promoter is easily achieved, and the technology has high commercial value.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to a method capable of capturing promoters from plants in batches. This method utilizes the "DNA-protein factor" complex formed in the promoter region when plant genes are transcribed, through improved chromosomal immunity Co-precipitation technique, using antibodies specific for protein factors in the complex, to isolate unknown gene promoter fragments from plant genomes. Background technique [0002] In the process of plant growth and development, the regulatory sequence of the gene, namely the promoter, plays a decisive role in the formation and growth of the specific tissue structure of the plant in different growth stages, as well as the stress response to external biotic and abiotic factors. The conserved sequence in the promoter region is the target of transcription factors, and the specific combination of th...

AI Technical Summary

Problems solved by technology

However, compared with other organisms, the gene discovery of plants is still quite limited in terms of quantity and quality, which is far from meeting the needs of crop molecular breeding.

In particular, the research and discovery of promoters are mostly based on the analysis, understanding, isolation and expression of individual genes, making it difficult to achieve large-scale and systematic development

[0005] There are also some methods for finding promoters in plant genes in the prior art. However, the methods in the prior art often estimate the possible existence of promoters through software calculations, and cannot directly obtain a large number of promoters at one time.

Images