Method for tracing pork through gene fluorescence labeling

Abstract

The invention discloses a method for tracing pork through gene fluorescence labeling. The method comprises the following steps: step 1, extracting genome DNA (Deoxyribonucleic Acid) in tissues of pig individuals; step 2, carrying out fluorescence labeling on four types of dideoxynucleotide of the genome DNA respectively; step 3, carrying out electrophoresis on the genome DNA to mutually separate DNA molecules with different lengths; labeling the DNA molecules respectively; step 4, irradiating with ultraviolet rays, wherein the DNA molecules of the four types of labeled dideoxynucleotide emit fluorescent light of different wavelengths; DNA molecule sequences can be distinguished by analyzing light spectrums of the fluorescent light; step 5, grouping and compiling the DNA molecule sequences to form DNA barcodes corresponding to the pig individuals by combining gene types corresponding to the DNA molecules, which are generated through the electrophoresis in step 3, of the four types of dideoxynucleotide. According to the method disclosed by the invention, pork tracing detection is carried out in links including production, slaughtering, processing, sales and the like of pork; manual intervention factors are effectively avoided and the safety is ensured when people eat the pork.

Description

technical field [0001] The invention relates to a method for traceability of pork, in particular to a method for traceability of pork by gene fluorescent labeling. Background technique [0002] With the improvement of living standards and health awareness, people pay more and more attention to food safety issues. As an important source of protein in human diet, pork products have become a global hot issue on how to ensure their safety and sanitation and protect the health and life safety of consumers. Governments around the world have adopted a series of technical measures to strengthen the safety management of meat products in order to minimize the occurrence of food safety incidents. Major cities in my country have also established traceability systems for meat products. Through the traceability management of meat products, consumers can be provided with accurate and detailed information about products, which is conducive to timely discovery of unsafe hidden dangers in va...

AI Technical Summary

Problems solved by technology

[0003] However, the current labeling technology used in the pork product traceability system has disadvantages such as label loss, record travel, unclear marking patterns, and labels are easy to be changed manually.