Method for tracing pork through gene fluorescence labeling

A fluorescent labeling and pork technology, which is applied in the field of pork traceability, can solve the problems of unclear marking patterns, label loss, and easy manual replacement of labels, so as to avoid intervention factors and ensure safety.

Inactive Publication Date: 2017-08-22
合肥岭牧农产品有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current labeling technology used in the pork product traceability system has disadvanta

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] The present invention is further illustrated below by means of examples, but the present invention is not limited to the scope of the examples.

[0020] Step 1, extracting the genomic DNA in the pig individual tissue;

[0021] Step 2, fluorescently labeling the four dideoxynucleotides of the genomic DNA respectively;

[0022] Step 3, performing electrophoresis on the genomic DNA to separate DNA molecules of different lengths from each other, and labeling the DNA molecules respectively;

[0023] Step 4, using ultraviolet radiation, the DNA molecules of the four labeled dideoxynucleotides emit fluorescence of different wavelengths, and the DNA molecular sequence can be distinguished by analyzing the fluorescence spectrum;

[0024] Step five, combining the genotypes corresponding to the four dideoxynucleotide DNA molecules produced by electrophoresis in step three, grouping and compiling the DNA molecular sequences into DNA barcodes corresponding to the individual pigs. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for tracing pork through gene fluorescence labeling. The method comprises the following steps: step 1, extracting genome DNA (Deoxyribonucleic Acid) in tissues of pig individuals; step 2, carrying out fluorescence labeling on four types of dideoxynucleotide of the genome DNA respectively; step 3, carrying out electrophoresis on the genome DNA to mutually separate DNA molecules with different lengths; labeling the DNA molecules respectively; step 4, irradiating with ultraviolet rays, wherein the DNA molecules of the four types of labeled dideoxynucleotide emit fluorescent light of different wavelengths; DNA molecule sequences can be distinguished by analyzing light spectrums of the fluorescent light; step 5, grouping and compiling the DNA molecule sequences to form DNA barcodes corresponding to the pig individuals by combining gene types corresponding to the DNA molecules, which are generated through the electrophoresis in step 3, of the four types of dideoxynucleotide. According to the method disclosed by the invention, pork tracing detection is carried out in links including production, slaughtering, processing, sales and the like of pork; manual intervention factors are effectively avoided and the safety is ensured when people eat the pork.

Description

technical field [0001] The invention relates to a method for traceability of pork, in particular to a method for traceability of pork by gene fluorescent labeling. Background technique [0002] With the improvement of living standards and health awareness, people pay more and more attention to food safety issues. As an important source of protein in human diet, pork products have become a global hot issue on how to ensure their safety and sanitation and protect the health and life safety of consumers. Governments around the world have adopted a series of technical measures to strengthen the safety management of meat products in order to minimize the occurrence of food safety incidents. Major cities in my country have also established traceability systems for meat products. Through the traceability management of meat products, consumers can be provided with accurate and detailed information about products, which is conducive to timely discovery of unsafe hidden dangers in va...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6439
Inventor 许伟
Owner 合肥岭牧农产品有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap