Method for extracting cellulose compound enzyme from agricultural by-products
A technology for cellulose composite enzymes and agricultural by-products, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, enzymes, etc., can solve the problem of being unsuitable for large-scale industrial production, reducing cellulase production, and low cellulase efficiency. and other problems, to achieve the effect of being conducive to resource recycling, good repeatability and simple operation
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example 1
[0019] The chaff of Grifola frondosa after producing two crops of fruiting bodies was selected and dried at a constant temperature of 65°C for 8 hours. Fresh bran was added to the dried chaff with a mass ratio of 1:1.5, the ratio of material to water was 1:1, and the pH was adjusted to 7.2. Nutritious comprehensive microbial culture medium. The Bacillus subtilis was activated, cultured at a constant temperature of 37°C for 48 hours in a 1% inoculum culture medium, and a cellulase-rich fermentation product was obtained. Add physiological saline to the fermentation product, shake the glass bead flask for 15 minutes, centrifuge at 5000rpm / min for 10 minutes, take the supernatant, add saturated ammonium sulfate to precipitate the protein, ultrafilter, freeze and vacuum-dry the enzyme solution to obtain the cellulose compound Enzyme powder. The obtained cellulose compound enzyme includes cellulase, xylanase and lignin peroxidase.
example 2
[0021] The chaff of Grifola frondosa after two crops of fruiting bodies was selected and dried at a constant temperature of 80°C for 6 hours, fresh bran was added to the dried chaff with a mass ratio of 1:2, the ratio of material to water was 1:1.25, and the pH was adjusted to 7.2 to produce Nutritious comprehensive microbial culture medium. Activate Bacillus subtilis, inoculate it into the culture medium prepared according to the step of 2% inoculum, and cultivate it at a constant temperature of 32°C for 48 hours to obtain a cellulase-rich fermentation product. Add physiological saline to the fermentation product, shake the glass bead flask for 25 minutes, centrifuge at 3500rpm / min for 20 minutes, take the supernatant, add saturated ammonium sulfate to precipitate protein, ultrafilter, freeze and vacuum-dry the enzyme solution to obtain cellulose complex enzyme powder. The obtained cellulose compound enzyme includes cellulase, xylanase and lignin peroxidase.
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