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Determination method for para-hydroxybenzoate alkyl esters in mouth cigarette

A p-hydroxybenzoic acid and snus technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of simple pretreatment steps, low detection limit and high purification efficiency

Active Publication Date: 2017-08-18
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Determination of Nepalgold Esters in Snuff Using Convergence Chromatography Tandem Mass Spectrometry, No Related Reports So far

Method used

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  • Determination method for para-hydroxybenzoate alkyl esters in mouth cigarette
  • Determination method for para-hydroxybenzoate alkyl esters in mouth cigarette
  • Determination method for para-hydroxybenzoate alkyl esters in mouth cigarette

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0036] Example: Determination of parabens in snus.

[0037] 1) Sample pretreatment

[0038]Cut open the package bag of snus, accurately weigh about 1g of sample into a 50mL centrifuge tube, add 0.1mL of 10000ng / mL internal standard solution (deuterated methylparaben), and then add 10mL of methanol, (The final internal standard concentration is 100ng / mL), ultrasonically extract for 30min in an ultrasonic wave, and centrifuge for 2min at a speed of 10,000 rpm in a centrifuge.

[0039] Take 1~1.5mL of the supernatant in the centrifuge tube to contain QuECHERS reagent (25mg PSA, 7.5mg Carbon, 150mgMgSO 4 ) in a centrifuge tube, vortexed for 1-2min, centrifuged in a centrifuge at 10,000 rpm for 1min, took the supernatant, filtered it through a 0.22µm nylon membrane, and determined it by ultra-high performance convergence chromatography tandem mass spectrometry .

[0040] 2) Using ACQUITY UPC 2 -MS / MS (Waters, USA) was used to determine the samples. Analysis conditions are: Chr...

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Abstract

The invention discloses a determination method for para-hydroxybenzoate alkyl esters in mouth cigarette. The determination method is characterized by comprising the following steps: extracting a sample by adopting a solution extraction method; purifying the sample by selecting QuECHERS (Quick, Easy, Cheap, Effective, Rugged and Safe) reagent; determining the sample through an ultraperformance convergence chromatography-tandem mass spectrometry technology. The determination method disclosed by the invention has the advantages that pretreatment steps are relatively simple, and used organic solvents are fewer; the dosage of the organic solvents used in a determination process is also small, determination of various components can be realized within three minutes, and the determination efficiency is high; compared with liquid chromatography, according to the determination method, the detection limit is low, the determination method is more suitable for determination of trace components, and the determination method for the para-hydroxybenzoate alkyl esters in the mouth cigarette is relative green, efficient and accurate.

Description

technical field [0001] The invention belongs to the field of physical and chemical analysis of tobacco product additives, in particular to a method suitable for the determination of p-hydroxybenzoate substances in snus. Background technique [0002] Parabens, also known as Nepal gold esters, include methyl esters, ethyl esters, propyl esters, etc., which are broad-spectrum and highly effective preservatives. At present, it is widely used in food, cosmetics, furniture, textiles and other daily chemical fields, and its existence is even detected in baby products (such as chewing gum). Parabens have a phenolic hydroxyl structure and have good antibacterial effects in the pH range of 3-8. In practical applications, it is rare to use one kind of ester alone, and it is mostly mixed use of several kinds of esters. [0003] In the past, studies believed that the toxicity of Nepalese gold esters was not large, but in 1998, Routledge et al first discovered that Nepalese gold esters ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 王颖邓惠敏边照阳杨飞刘珊珊李中皓张威唐纲岭
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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