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Preparation method of naringenin molecular imprinting electrochemical sensor

A technology of molecular imprinting and naringenin, applied in the direction of material electrochemical variables, etc., can solve the problems of poor regeneration and reversibility, high detection limit, slow electron transfer speed and response, and achieve high affinity and selectivity, response Improved effect

Inactive Publication Date: 2017-08-18
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the thickness of the imprinted film prepared by the traditional imprinting method is difficult to control, and the high cross-linking degree makes the electron transfer speed and response slow, the detection limit is high, and the regeneration and reversibility are poor, which affects the application of molecular imprinting technology in electrochemical sensors.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Preparation of nano-silver / carbon nanotube modification solution: In the reactor, add deionized water: 8 mL, silver nitrate: 0.3 g, carbon nanotube oxide: 0.6 g, ultrasonically for 20 minutes at room temperature, and disperse evenly. 20% hydrazine hydrate: 1.1 mL, stirred and reacted for 20 minutes, left overnight to obtain nano-silver / carbon nanotube modification solution;

[0023] (2) Preparation of nano-silver / carbon nanotube modified electrode: Polish the glassy carbon electrode with 0.2 μm and 0.01 μm polishing powder in turn, then ultrasonically clean it with twice-distilled water, wash with ethanol, and dry it, and place it on the surface of the glassy carbon electrode. Add 13 μL of nano-gold graphene modification solution dropwise, place it under an infrared lamp, and evaporate the solvent to obtain a nano-silver / carbon nanotube modified electrode;

[0024] (3) Preparation of naringenin molecularly imprinted polymer: In the reactor, add ethanol: 7.0 mL, dipe...

Embodiment 2

[0027] (1) Preparation of nano-silver / carbon nanotube modification solution: In the reactor, add deionized water: 7.8 mL, silver nitrate: 0.2 g, carbon nanotube oxide: 0.5 g, and ultrasonically disperse for 20 minutes at room temperature. 20% hydrazine hydrate: 1.5 mL, stirred and reacted for 20 minutes, left overnight to obtain nano-silver / carbon nanotube modification solution;

[0028] (2) Preparation of nano-silver / carbon nanotube modified electrode: Polish the glassy carbon electrode with 0.2 μm and 0.01 μm polishing powder in turn, then ultrasonically clean it with twice-distilled water, wash with ethanol, and dry it, and place it on the surface of the glassy carbon electrode. Add 14 μL of nano-gold graphene modification solution dropwise, place it under an infrared lamp, and evaporate the solvent to obtain a nano-silver / carbon nanotube modified electrode;

[0029] (3) Preparation of naringenin molecularly imprinted polymer: In the reactor, add ethanol: 6.3mL, dipentaeryt...

Embodiment 3

[0032] (1) Preparation of nano-silver / carbon nanotube modification solution: In the reactor, add deionized water: 8.4mL, silver nitrate: 0.3g, carbon nanotube oxide: 0.3g, ultrasonically 20min at room temperature, and disperse evenly. 20% hydrazine hydrate: 1.0 mL, stirred and reacted for 20 minutes, left overnight to obtain nano-silver / carbon nanotube modification solution;

[0033] (2) Preparation of nano-silver / carbon nanotube modified electrode: Polish the glassy carbon electrode with 0.2 μm and 0.01 μm polishing powder in turn, then ultrasonically clean it with twice-distilled water, wash with ethanol, and dry it, and place it on the surface of the glassy carbon electrode. Add 15 μL of nano-gold graphene modification solution dropwise, place it under an infrared lamp, and evaporate the solvent to obtain a nano-silver / carbon nanotube modified electrode;

[0034] (3) Preparation of naringenin molecularly imprinted polymer: In the reactor, add ethanol: 7.6 mL, dipentaerythri...

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Abstract

The invention discloses a preparation method of a naringenin molecular imprinting electrochemical sensor. The preparation method is characterized in that firstly, a glassy carbon electrode is modified with a nano silver / carbon nanotube, and a nano silver / carbon nanotube modified electrode is obtained; 50%-60% of ethanol, 15%-20% of dipentaerythritol triacrylate, 4%-8% of methacrylic acid, 12%-18% of 1-vinyl-3-ethyl imidazole tetrafluoroborate, 1.0%-2.0% of azo-bis-iso-heptonitrile and 3.0%-6.0% of naringenin are added to a reactor, stirred, dissolved and stirred to react at the temperature of 65+ / -2 DEG C for 12-15 h in an oxygen-free atmosphere, template molecules are removed through a methanol and acetic acid mixed solution, and naringenin molecular imprinting polymers are obtained; the naringenin molecular imprinting polymers are dispensed to the nano silver / carbon nanotube modified electrode, and the naringenin molecular imprinting electrochemical sensor is obtained. The sensor has high affinity, selectivity and sensitivity and good specificity, can realize rapid detection and can be repeatedly used.

Description

technical field [0001] The present invention relates to a preparation method of a molecularly imprinted sensor and the technical field of rapid detection application, in particular to a preparation method of a molecularly imprinted electrochemical sensor for naringenin, which is used to detect naringenin in medicines, foods, and biological samples . Background technique [0002] Naringenin (scientific name is 4',5,7-trihydroxyflavone) has the molecular formula C 15 h 12 o 5 , with a relative molecular mass of 272.25, naringenin is a white needle-like crystal, soluble in acetone, ethanol, ether and benzene, almost insoluble in water. The reaction of magnesium hydrochloride powder is cherry red, the reaction of sodium tetrahydroborate is reddish purple, and the reaction of molish is negative. Naringenin is the aglycone of naringin, which belongs to dihydroflavonoids, has antibacterial, anti-inflammatory, free radical scavenging, anti-oxidation, cough and expectorant, blood...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/26
CPCG01N27/26G01N27/30
Inventor 李慧芝赵淑英卢燕
Owner UNIV OF JINAN
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