Kit for rapidly extracting RNA of influenza viruses
A kit and rapid technology, applied in the field of molecular biology detection, can solve the problems of long purification cycle, complex extraction steps, and unfavorable customers to quickly extract and purify RNA.
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Embodiment 1
[0021] The final concentration of the virus lysis binding solution is composed of: 20mM sucrose, 10mM Tris-HCl hydrochloride (Tris-HCl), 14% (V / V) polyether alcohol, the solvent is high-pressure sterilized water, and the virus lysis combination The pH value of the solution is 7.0. The method of using the virus lysis binding solution for nucleic acid detection: take a 1.5ml centrifuge tube without RNase, add 200 μl of the virus lysis binding solution to the tube, and then add 200 μl of fresh influenza samples, shake and mix Let stand at room temperature for 5 minutes.
[0022] The sample volume extracted by QIAamp Viral RNA Mini Kit was 200 µl, and the elution volume was 100 µl.
[0023] After the extraction is completed, take 5 µl of the above nucleic acid RNA extraction solution as a template, and use Daan Gene’s Influenza A Virus Nucleic Acid Detection Kit (one-step PCR fluorescence method) for detection. The detection results are shown in Table 1 below and attached figure ...
Embodiment 2
[0028] The experimental method is the same as in Example 1, the only difference is that the final concentration of the virus lysis binding solution is composed of: 30mM sucrose, 30mM Tris-HCl, 12% (V / V) polyether alcohol, the solvent is autoclaved water, and the virus lysis binding solution The pH value is 6.5.
Embodiment 3
[0030] The experimental method is the same as in Example 1, the only difference is that the final concentration of the virus lysis binding solution is composed of: 50mM sucrose, 50mM Tris-HCl, 10% (V / V) polyether alcohol, the solvent is autoclaved water, and the virus lysis binding solution The pH value is 7.5.
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