A kind of Streptomyces aflatoxin and bacterial agent and application thereof

A technology of Streptomyces flavonoids and bacterial agent, which is applied in the application, fungicide, bacteria and other directions to achieve the effect of reducing plant diseases, reducing the number of pathogens, and promoting rot.

Active Publication Date: 2020-08-04
陕西博秦生物工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the biological control of canker sores mainly focuses on Trichoderma and bacteria. There are few reports on the use of actinomycetes to inhibit Sclerotium rolfsii. Report on Sclerotia Formation and Inhibition of Sclerotia Germination in Soil

Method used

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  • A kind of Streptomyces aflatoxin and bacterial agent and application thereof
  • A kind of Streptomyces aflatoxin and bacterial agent and application thereof
  • A kind of Streptomyces aflatoxin and bacterial agent and application thereof

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Effect test

preparation example Construction

[0030] Preparation of Streptomyces aflavitus preparation: Solid-state fermentation of Streptomyces aflavitus was carried out to prepare spore powder, and the number of viable bacteria in the obtained bacterial preparation was 3.3×10 11 cfu / g, its active ingredients are Streptomyces aflatoxin 25 viable spores and the secondary metabolites of Streptomyces aflatoxin 25.

[0031] Preparation of Sclerotinum thallus powder: Pick appropriate amount of Sclerotinia mycelia activated on the slope of PDA with a bamboo stick, inoculate into a 500mL shaker flask containing 200mL Cha's liquid medium, 25°C, 160r· min -1 Shaker shaking culture 5d. The mycelium was collected by filtration with double-layer gauze, rinsed with deionized water several times until there was no residual culture solution, dried at 40°C, ground into a fine powder with a mortar, and passed through a 0.25mm sieve.

[0032] Preparation of target bacterium pathogenic bacteria cake: pick the neat mycelia of Sclerotium rol...

Embodiment 1

[0037] Inhibition of mycelial growth by Streptomyces flavotricini 25

[0038] (1) Test method:

[0039] Mix the cell-free fermentation filtrate of Streptomyces flavus (stock solution, 5, 10 times dilution in sterile water) and the PDA medium cooled to about 50°C in a volume ratio of 1:4, and then pour it on a plate to obtain a final dilution of 5 , 25 and 50 times the fermentation filtrate PDA plate; pick the neat Sclerotium rolfsii agar piece in the center of the plate with a sterilized bamboo stick, with the bacterium face up, and repeat 3 times for each treatment; use sterile water as a control. Cultivate at 28°C, measure the colony diameter with the cross method after 24h and 48h respectively, and calculate the bacteriostatic rate (Inhibition Rate of Mycelia Growth, IR MG )

[0040]

[0041] Where: IR MG is the antibacterial rate of antagonistic bacteria cell-free fermentation filtrate to mycelia growth, D CK 、D t are the colony diameters of control and treatment, ...

Embodiment 2

[0047] Inhibition of Sclerotia Formation by Streptomyces flavotricini 25

[0048] (1) Test method:

[0049] After completing the measurement of Streptomyces aflavitinus to the mycelia growth inhibition rate, the petri dish was placed at room temperature and cultivated for 20 days, and the produced sclerotium was scraped off and counted, and the inhibition rate (Inhibition Rate of SclectiaFormation, IR SF ) is calculated according to the following formula:

[0050]

[0051] Where: IR SF is the inhibition rate of sclerotia formation, N CK , N t are the number of sclerotia formed in the control and treatment dishes, respectively.

[0052] (2) Test results

[0053] From Table 2 and image 3 It can be seen that when cultured for 20 days, the formation of Sclerotinia sclerotia in different dilution ratios of Streptomyces aflatoxin all inhibited the formation of sclerotia (P<0.01). The inhibitory effect of Streptomyces aflavitinus was good. When diluted 5, 25 and 50 times, ...

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Abstract

The invention specifically discloses a Streptomyces flavotricini 25, which was preserved in the China Center for Type Culture Collection on March 14, 2016, with a preservation number of CCTCC NO: M 2016121. The active ingredient of the streptomyces aflavites inoculant is live spores of the streptomyces aflavites and / or secondary metabolites of the streptomyces aflavites. It also discloses the application of Streptomyces flavitinus and its fungal agent in preventing and treating white silkworm. The Streptomyces aflavous of the invention can produce active substances against Sclerotinia sclerotiorum, can dissolve the cell wall of Sclerotinia sclerotium, and simultaneously produce enzyme-soluble antibacterial effect on pathogenic hyphae and antagonism of active substances. The application of Streptomyces aflatoxin in the non-sterilized soil can significantly promote the rot of the neat Sclerotia sclerotia in the soil, reduce the germination activity of the unrotted sclerotia, reduce the number of pathogens from the source, and reduce the damage of white silkworm.

Description

technical field [0001] The invention relates to the field of biological control of plant diseases, in particular to a streptomyces aflavitinus and bacterial agent and application thereof. Background technique [0002] White silkworm is a common plant soil-borne fungal disease caused by Sclerotium rolfsii. It mainly occurs at the base of crop stems. When the soil is relatively moist, a white mycelium layer will form on the surface of the diseased plant and the surrounding soil , and then develop into a sclerotium resembling a rapeseed. In the later stages of the disease, stem base rot blocks water and nutrient transport, causing the crop to dry out and die. There are more than 500 kinds of hosts of Sclerotinia sclerotiorum, which are extremely harmful to agricultural production. Sclerotia sclerotiorum can produce sclerotia, which improves the resistance of pathogenic bacteria to pesticides and other control measures, and increases the difficulty of the control of white silk...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/28A01P3/00C12R1/465
CPCA01N63/00A01N63/10C12N1/205C12R2001/465
Inventor 薛泉宏李玉龙来航线
Owner 陕西博秦生物工程有限公司
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