Biological organic fertilizer produced from abamectin fermentation waste residue and preparation method thereof
A bio-organic fertilizer and abamectin technology, applied in the field of agricultural biology, can solve the problems of inability to produce bio-organic fertilizers, inability to degrade abamectin fermentation residues, etc., and achieve shortening of decomposing time, speeding up degradation speed, and accelerating decomposing and aging. Effect
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Embodiment 1
[0019] Embodiment 1. preparation of Abamectin degrading bacterial agent
[0020] 1) The abamectin-degrading bacteria AZ11 slant-preserved test tube seeds were inoculated into LB liquid medium, and shaken at 40-45°C until the logarithmic growth phase;
[0021] 2) Inoculate the cultured bacterial solution into a 10L seed fermenter with 10% inoculation amount, and cultivate it at 40-45°C until the logarithmic growth phase. The cultivation and formula of the seed fermenter are: corn flour 2%; bean cake powder 1 %; Glucose 0.5%; K 2 HPO 4 0.4%; MgSO 4 ·7H 2 O 0.02%; CaCO 3 0.2%; pH 7.2-7.5;
[0022] 3) Inoculate the fermented liquid of the seed fermenter with 10% inoculum into the production fermenter for fermentation production. The medium formula used in the fermentation production is: corn flour 3%; bean cake flour 2%; CaCO 3 0.5%;K 2 HPO 4 0.2%; ZnSO 4 ·5H 2 O 0.05%; MgSO 4 ·7H 2 O 0.05%; pH 7.2;
[0023] The fermentation production process conditions...
Embodiment 2
[0025] Embodiment 2. Abamectin fermentation waste residue bio-organic fertilizer preparation
[0026] According to the weight ratio of 50% of abamectin waste residue, 40% of straw, and 10% of chicken manure, the composition of the pile is prepared, and water is added until the water content is 60%, and stirred evenly; refer to the size of the fermentation site, and the length of the pile is 10.0 meters, and the width is 8.0 meters. , with a pile height of 1.5 meters; after the pile temperature rises to 40°C, inoculate the degrading bacteria agent weighing 1% of the total weight of the raw materials (dry weight) on average twice, with an interval of 4 days; use fully automatic fermentation every 3 days Turn the pile once with the mixer, ventilate for 1.5 hours every 12 hours, and ferment for 24 days. When the pile temperature drops below 40°C, the high-temperature fermentation process is over, and the residue of abamectin in the pile is detected; at this time, the fermentation m...
Embodiment 3
[0027] Embodiment 3 The screening of the preparation process condition of bio-organic fertilizer of the present invention, preferred test
[0028] The test method is to change one process condition, fix the other conditions (not necessarily the optimal point), conduct a fermentation test, and evaluate the optimal process parameters by measuring the degradation rate of abamectin waste residue.
[0029] 1) Determination of the inoculum amount of degrading bacteria
[0030]After the stacking temperature naturally rises to 40°C, inoculate the degrading bacteria agent at 0.1%, 0.5%, and 1% inoculum respectively, and set a control without inoculation, turn the stack once every 3 days with a fully automatic fermentation mixer, and detect Avi The remaining amount of mycocin is fermented until the pile temperature is below 40°C.
[0031] The results showed that adding the degrading bacteria agent could significantly improve the degradation of abamectin in the pile. At the end ...
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