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Botrytis crust fungi strain and method for rapidly and artificially promoting biological crust culturing by adopting exopolysaccharides of botrytis crust fungi strain

A botrytis and exopolysaccharide technology, which is applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low survival rate, high cost, and strong evaporation of afforestation

Inactive Publication Date: 2017-08-18
YANAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main methods of sand prevention and sand fixation are physical sand fixation, chemical sand fixation and vegetation cultivation, but the cost is relatively high, and the precipitation in arid and semi-arid areas is insufficient, evaporation is strong, and the survival rate of afforestation is low

Method used

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  • Botrytis crust fungi strain and method for rapidly and artificially promoting biological crust culturing by adopting exopolysaccharides of botrytis crust fungi strain
  • Botrytis crust fungi strain and method for rapidly and artificially promoting biological crust culturing by adopting exopolysaccharides of botrytis crust fungi strain
  • Botrytis crust fungi strain and method for rapidly and artificially promoting biological crust culturing by adopting exopolysaccharides of botrytis crust fungi strain

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Relational model of embodiment 1 bacterial strain exopolysaccharide content and fermentation broth viscosity

[0038] (1) Strain screening and identification

[0039] Strains were screened using the high-fold dilution method.

[0040] Observation and identification of strain colony morphology: single colony was cultivated on PDA medium by single hyphae picking method, and the density, surface shape, size, color, texture, growth speed, edge shape, etc. of the aerial hyphae of the colony were recorded. Using insert culture method, observe the microscopic morphological characteristics and classify and identify the isolated fungi, and classify and identify according to the Ainsworth classification system, and identify the genus.

[0041] (2) Extraction, purification and identification of polysaccharides

[0042] Take the activated strain, put it into a 500mL Erlenmeyer flask containing 200ml of liquid fermentation medium, culture it with shaking at 28°C for 3 days, measur...

Embodiment 2

[0045] Embodiment 2 thin-layer chromatography (TLC) detects

[0046] (1) Strain screening and identification

[0047] Strains were screened using the high-fold dilution method.

[0048] Observation and identification of strain colony morphology: single colony was cultivated on PDA medium by single hyphae picking method, and the density, surface shape, size, color, texture, growth speed, edge shape, etc. of the aerial hyphae of the colony were recorded. Using insert culture method, observe the microscopic morphological characteristics and classify and identify the isolated fungi, and classify and identify according to the Ainsworth classification system, and identify the genus.

[0049] (2) Extraction, purification and identification of polysaccharides

[0050] Take the activated strain, put it into a 500mL Erlenmeyer flask containing 200ml of liquid fermentation medium, culture it with shaking at 28°C for 3 days, measure the viscosity with a NDJ-79 rotary viscometer, and use...

Embodiment 3

[0057] Infrared spectrum analysis of embodiment 3 exopolysaccharide

[0058] (1) Strain screening and identification

[0059] Strains were screened using the high-fold dilution method.

[0060] Observation and identification of strain colony morphology: single colony was cultivated on PDA medium by single hyphae picking method, and the density, surface shape, size, color, texture, growth speed, edge shape, etc. of the aerial hyphae of the colony were recorded. Using insert culture method, observe the microscopic morphological characteristics and classify and identify the isolated fungi, and classify and identify according to the Ainsworth classification system, and identify the genus.

[0061] (2) Extraction, purification and identification of polysaccharides

[0062] Take the activated strain, put it into a 500mL Erlenmeyer flask containing 200ml of liquid fermentation medium, culture it with shaking at 28°C for 3 days, measure the viscosity with a NDJ-79 rotary viscometer,...

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Abstract

The invention provides a botrytis crust fungi strain and a method for rapidly and artificially promoting biological crust culturing by adopting exopolysaccharides of the botrytis crust fungi strain. The botrytis strain is prepared in CGMCC on May 17, 2012, and is assigned with the accession number of CGMCC NO.3.379. The method for rapidly and artificially promoting biological crust culturing by adopting exopolysaccharides prepared by fermenting the strain comprises the following steps: selecting and identifying the strain; extracting polysaccharide, carrying out purifying, and carrying out identifying; carrying out water retention experiment; and carrying out crusting experiment, and thus the biological crust culturing is rapidly and artificially promoted. The invention provides a novel large-scale production path for rapidly and artificially promoting the biological crust culturing by adopting the exopolysaccharides of the crust fungi; the mycelia composed of a large number of hyphae have the great winding and embedding effects for sand grains, have the effect of improving stabilization and prevention for sand better than that obtained only depending on the adhesion function of the viscous matter such as polyacrylamide and sodium alginate, and have no adverse effects on the development of crusted organisms, and thus the application prospect is good.

Description

technical field [0001] The invention relates to the production of exopolysaccharides of crustacean fungi, in particular to a method for quickly and artificially promoting the cultivation of biological crusts by a Botrytis crustacean fungus strain and its exopolysaccharides. Background technique [0002] A good symbiotic relationship is formed among microorganisms, short-lived plants and various kinds of lichens, mosses and algae, together with their inherent characteristics of secreting extracellular mucopolysaccharides, it forms a good micro-ecosystem as a whole. Microorganisms are The pioneer species of biological soil crusts is also the dominant component in the early stage of biological soil crust succession, which can provide a certain material basis for plants to promote their growth and development. Biological crust field experiments have proved that under the joint action of other short-lived plants and rich lichens, mosses and algae plants, the thicker the crust for...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01B79/02C12R1/645
CPCA01B79/02C12N1/145C12R2001/645
Inventor 邓振山
Owner YANAN UNIV
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