A kind of nano gel slant culture medium and preparation method thereof
A slant culture medium and nano-gel technology, applied in the field of nano-gel slant culture medium and its preparation, can solve the problems such as affecting the growth of bacterial liquid, nutrient balance, too large difference in thickness between the upper and lower surfaces of the slant, affecting test accuracy, etc. The effect of avoiding repeated tests, delaying the setting time and shortening the test time
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Embodiment 1
[0019] A nanogel slant culture medium, used for the cultivation of Staphylococcus aureus, specifically includes the following components: glucose 20g, peptone 15g, sodium chloride 5g, beef extract 0.5g, agar powder 7g, nanogel particles 6g, agar Sugar gel 7g; add distilled water to make up to 1L;
[0020] The nanogel particle is the preparation of zinc oxide-titanium dioxide nanocomposite material: dissolve zinc chloride in an acidic solution of dehydrated alcohol ten times the volume, react in a low-temperature plasma reactor for 10 minutes, add titanium dioxide powder and continue the reaction for 20 minutes, The reaction temperature is -12°C. After the reaction is completed, take it out and stir it sufficiently until the liquid phase is evenly dispersed; adjust the pH to 2, and then place it in a low-temperature plasma reactor at -5°C for more than 12 hours to form a uniform sol, then take it out and evaporate it It is dried and transformed into a gel to obtain nanogel part...
Embodiment 2
[0027] A nanogel slant medium, used for the cultivation of Staphylococcus aureus, specifically includes the following components: glucose 20g, peptone 15g, sodium chloride 5g, beef extract 0.5g, agar 11g, nanogel particles 4g, agarose Gel 5g; add distilled water to make up to 1L;
[0028] The nano gel particle is the preparation of zinc oxide-titanium dioxide nanocomposite material: dissolve zinc chloride in an acidic solution of dehydrated alcohol ten times the volume, react in a low-temperature plasma reactor for 20 minutes, add titanium dioxide powder to continue the reaction for 30 minutes, The reaction temperature is -6°C. After the reaction is completed, take it out and stir it sufficiently until the liquid phase is uniformly dispersed; adjust the pH to 2-3, then put it in a low-temperature plasma reactor and let it stand for more than 12 hours at 0°C to form a uniform sol, then take it out Evaporated and dried into a gel to obtain nanogel particles with a size of less t...
Embodiment 3
[0031] A kind of nano-gel slant culture medium, used for the cultivation of Staphylococcus aureus, specifically includes the following components: glucose 20g, peptone 15g, sodium chloride 5g, beef extract 0.5g, agar powder 9g, nano-gel particles 5g, agar Sugar gel 6g; add distilled water to make up to 1L;
[0032] The nano gel particle is the preparation of zinc oxide-titanium dioxide nanocomposite material: dissolve zinc chloride in an acidic solution of dehydrated alcohol ten times the volume, react in a low-temperature plasma reactor for 15 minutes, add titanium dioxide powder to continue the reaction for 25 minutes, The reaction temperature is -9°C. After the reaction is completed, take it out and stir it sufficiently until the liquid phase is uniformly dispersed; adjust the pH to 2.5, then place it in a low-temperature plasma reactor for more than 12 hours at -3°C to form a uniform sol, then take it out and evaporate it It is dried and transformed into a gel to obtain na...
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