Construction and application of H5 subtype avian influenza recombinant duck enteritis virus rDEVdeltaUL2-H5 strain

A duck enteritis virus, UL2-H5 technology, applied in the field of virology and veterinary biological products, can solve the problems of human infection, poultry industry blow, death and other problems

Inactive Publication Date: 2017-08-01
CHINA INST OF VETERINARY DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The highly pathogenic avian influenza prevailing in my country is mainly caused by the H5 subtype avian influenza virus, which not only brings a devastating blow to the poultry industry, but also repeatedly breaks through the interspecies barrier, causing human infection and death

Method used

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  • Construction and application of H5 subtype avian influenza recombinant duck enteritis virus rDEVdeltaUL2-H5 strain
  • Construction and application of H5 subtype avian influenza recombinant duck enteritis virus rDEVdeltaUL2-H5 strain
  • Construction and application of H5 subtype avian influenza recombinant duck enteritis virus rDEVdeltaUL2-H5 strain

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Experimental program
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Embodiment Construction

[0013] 1. Construction of recombinant virus

[0014] The recombinant virus of the present invention is the duck enteritis virus rDEVΔUL2-H5 strain expressing the H5 subtype avian influenza virus HA gene, and the virus construction method is as follows:

[0015] (1) carrier

[0016] The pMD-18T vector, used for cloning, sequencing and construction of transfer vectors, was purchased from Bao Biological Engineering (Dalian) Co., Ltd. The GFP reporter gene vector pT-GFP-gpt containing CMV promoter, polyA (see figure 1 ), constructed and preserved by the inventor's laboratory.

[0017] (2) Primer design

[0018] The following primers were designed and synthesized according to the DEV sequence published on GenBank:

[0019] Table 1 The relevant primer information involved in the construction of the present invention

[0020]

[0021]

[0022] (3) Construction of GFP-tagged duck enteritis virus rDEVΔUL2-GFP

[0023] 1) Construction of transfer vector pT-UL2-GFP-gpt

[00...

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Abstract

The invention relates to construction and application of H5 subtype avian influenza recombinant duck enteritis virus rDEVdeltaUL2-H5 strain. A strain of recombinant duck enteritis virus rDEVdeltaUL2-H5 is designed and built, is the recombinant duck enteritis virus expressing 5 subtype avian influenza virus hemagglutinin (HA) gene, and can be used for preparing survive vaccine and preventing duck plague and H5 subtype avian influenza of chickens and water birds; missing UL2 genes and carrying green fluorescent label in construction process, a recombinant duck enteritis virus carrier (rDEVdeltaUL2-GFP) can be applied to express exogenous genes through a homologous recombination method, and lay the foundation for building new recombinant vaccine for resisting the duck plague and other pathogens taking the duck enteritis virus as a carrier.

Description

technical field [0001] The invention relates to the construction and application of H5 subtype avian influenza recombinant duck enteritis virus rDEVΔUL2-H5 strain and belongs to the fields of virology and veterinary biological products. Background technique [0002] Duck enteritis virus (DEV), also known as duck plague virus (Duck Plague virus), can infect ducks, geese and other Anseriformes birds, causing blood vessel damage, tissue bleeding, digestive tract mucosal damage, and lymphoid organ damage Duck viral enteritis (DVE), or duck plague (DP) (Sandhu and Metwally, 2008). Since it was first discovered in 1923 (Baudet, 1923), the disease has successively occurred in countries such as France, India, Belgium, the United Kingdom, Thailand, and Canada (Yin Zhen and Liu Jinghua, 1997), and the scope of the epidemic and the types of infected poultry have gradually expanded. According to the trend, at least 48 species of birds in Anseriformes are susceptible to DEV (Kaleta et a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01A61K39/295A61K39/245A61K39/145A61P31/16A61P31/22C12R1/93
CPCA61K39/12A61K2039/5256A61K2039/552A61K2039/70C12N7/00C12N2710/16321C12N2710/16334C12N2760/16122C12N2760/16134
Inventor 杨承槐李俊平侯力丹刘丹毛娅卿李岭李启红李慧姣王乐元
Owner CHINA INST OF VETERINARY DRUG CONTROL
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