A kind of Acacia open type tissue culture method

An open, tissue culture technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of poor effect, difficult to obtain, high cost, achieve good bacteriostatic effect, simple ingredients, easy to buy Effect

Active Publication Date: 2019-10-22
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research has not yet established a perfect technical system. The main problems are: the bacteriostatic agents used are complex in composition, difficult to obtain (artificial extraction or high cost), and poor effect, which is not conducive to popularization and application.

Method used

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  • A kind of Acacia open type tissue culture method
  • A kind of Acacia open type tissue culture method
  • A kind of Acacia open type tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1 material

[0040] Referring to the method of Shi Qiong et al. (2015), excellent individual plants were selected from the 18-year-old experimental forest of Acacia mara hybrida, Institute of Tropical Forestry, Chinese Academy of Forestry. , The branches with full axillary buds were collected from the annual ear-picking nursery as the research material.

[0041] 2 methods

[0042] Bud induction explants: In August, the full axillary buds of the current year in the Acacia mara hybrids were cut off, the leaves were cut off, soaked in washing powder water for 30 minutes, washed with a brush for 2 hours, and cut to a length of 2.0 to 3.0 cm. Stem segments with axillary buds.

[0043] 1) Screening of types and concentrations of bacteriostatic agents

[0044] Bacteriostatic agents: chlorothalonil, carbendazim. The two bacteriostatic agents were set at 4 concentrations: 0.2, 0.4, 0.6, 0.8g·L -1 . There were 8 treatments in total, each treatment was inoculated with 24 ste...

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Abstract

The invention discloses an auriculiformis open type tissue culture method. The method comprises the following steps: using an annual branch with plump axillary buds from April to October as an explant, disinfecting, and culturing in an open type shoot induction medium to obtain the axillary buds, performing open type test-tube rooting culture on the axillary buds in an open type rooting medium to obtain rooting seedlings; cutting the rooting seedlings into sections each having at least one bud as successive explants, and continuously culturing in the open type rooting medium for rooting for many generations to realize the aim of multiplication. A bacteriostatic agent, chlorothalonil, used by the method is simple in component, low in cost and easy to buy, and a good antibacterial effect can be realized by just using 0.2.L<-1>; by screening study on the explant type, the disinfection method, the explant collection season, the shoot induction medium and the like, the shoot inductivity is up to 99.54%; the rooting percentage is 97.62%, the multiplication multiple of rooting for many generations can reach 3.58, so as to completely meet the requirements of industrialized production.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to an open tissue culture method for Acacia. Background technique [0002] Asexual reproduction can maintain the excellent characters of its excellent mother plants to the greatest extent, which is beneficial to the fixation of the excellent characters of the excellent trees, thereby facilitating the rapid propagation and popularization of the improved varieties. Cuttings and tissue culture are the two most widely used techniques for rapid propagation of trees. Many tree species have been able to rapidly propagate a large number of seedlings through cutting technology. Although this technology is simple to operate and easy to master, it requires a large area of ​​land to build a panicle garden to provide cuttings. Nowadays, the serious shortage of land resources makes this technology The popularization and application of it is greatly restricted; in addition, there are...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 黄烈健王鸿曾炳山吴友军
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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