Yeast for synthesizing 3-indolebutyric acid and application of yeast in 3-indolebutyric acid preparation
A technology of indole butyric acid and yeast, which is applied in the direction of fermentation, fungi, and microorganism-based methods to achieve the effects of environmental friendliness, complex processes, and mild conditions
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0013] Saccharomyces sp. of the present invention, detailed separation and screening steps are as follows:
[0014] (1) Sample collection: Take the rhizosphere soil of plants with well-developed root system.
[0015] (2) Isolation of bacterial strains: Weigh 10g of the above soil sample and place it in a 250ml sterilized Erlenmeyer flask filled with 100ml of sterile water, place it in a shaker at 28°C for half an hour to obtain a suspension, and then gradually dilute the suspension with sterile water. diluted to 10 -3 , 10 -4 , 10 -5 The three dilutions are ready for use. Take 0.1ml of the above-mentioned diluted suspension and spread it on the solid PDA medium plate, spread 3 plates for each dilution, and culture it in a 28°C incubator for one week. After the grown strains are purified Do follow-up tests. The formula of solid PDA medium is as follows: 200 grams of potatoes, 20 grams of glucose, 15-20 grams of agar, 1000 ml of tap water, and natural pH.
[0016] (3) Scree...
Embodiment 2
[0022] The application of continuous culture of yeast with the function of synthesizing IBA to the synthesis of IBA.
[0023] (1) Inoculate a loop of yeast LY1 into 50 ml of liquid PDA medium from a slant storage tube, place it in a shaker at 28°C, shake at 150 rpm until the exponential phase, and use it as the primary seed liquid; the formula of the liquid PDA medium is as follows: Potato 200 grams, 20 grams of glucose, 1000 ml of tap water, natural pH,
[0024] (2) Take the seed solution obtained in step (1), inoculate it into the liquid PDA medium in a 20L fermenter with an inoculum volume of 5%, and culture it with aeration at 28°C until the exponential phase, as the secondary seed solution,
[0025] (3) Inoculate the secondary seed liquid into the YPDA medium of a 200L fermenter, cultivate to the stationary phase, and add 3-indoleacetic acid solution with a concentration of 0.8% by weight (3-indoleacetic acid is saturated with bicarbonate Dissolved in sodium solution or ...
Embodiment 3
[0028] The application of the bacterial strain with the function of synthesizing IBA to the IAA-producing microbial fermentation liquid to synthesize IBA.
[0029] (1) Microbial fermentation of IAA: Inoculate the IAA-producing strain Enterobacter LY6 (preservation number CGMCC NO 9539) into the medium (tryptone 0.8%, yeast extract 0.4%, NaCl 0.7%, glucose 2%, fructose 0.7%, L - 0.12% tryptophan, 0.02% sodium pyruvate, 0.01% copper sulfate, 1L water, pH 7.2), sterilized for 15 minutes after fermentation, and the sterilized fermentation broth was used for the synthesis of the yeast with the function of synthesizing IBA IBA.
[0030] (2) Preparation of yeast seed liquid: inoculate a ring of the yeast LY1 into 50 ml of liquid PDA medium from a slant preservation tube, place it in a shaker at 28°C, shake at 150 rpm until the exponential phase, and use it as the primary seed liquid; The primary seed solution was inoculated into the liquid PDA medium in a 20L fermenter with an inocu...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com