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Yeast for synthesizing 3-indole butyric acid and its application for preparing 3-indole butyric acid

A technology of indole butyric acid and yeast, applied in the direction of fermentation, fungi, microorganism-based methods, etc., to achieve the effect of solving complex process, mild conditions and environmental friendliness

Active Publication Date: 2020-06-02
SICHUAN LANYUE SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no relevant report on the preparation of IBA by microbial fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Saccharomyces sp. of the present invention, detailed separation and screening steps are as follows:

[0013] (1) Sample collection: Take the rhizosphere soil of plants with well-developed root system.

[0014] (2) Isolation of bacterial strains: Weigh 10g of the above soil sample and place it in a 250ml sterilized Erlenmeyer flask filled with 100ml of sterile water, place it in a shaker at 28°C for half an hour to obtain a suspension, and then gradually dilute the suspension with sterile water. diluted to 10 -3 , 10 -4 , 10 -5 The three dilutions are ready for use. Take 0.1ml of the above-mentioned diluted suspension and spread it on the solid PDA medium plate, spread 3 plates for each dilution, and culture it in a 28°C incubator for one week. After the grown strains are purified Do follow-up tests. The formula of the solid PDA medium is as follows: 200 grams of potatoes, 20 grams of glucose, 15-20 grams / liter of agar, 1000 milliliters of tap water, and natural pH. ...

Embodiment 2

[0021] The application of continuous culture of yeast with the function of synthesizing IBA to the synthesis of IBA.

[0022] (1) Inoculate a loop of yeast LY1 into 50 ml of liquid PDA medium from a slant storage tube, place it in a shaker at 28°C, shake at 150 rpm until the exponential phase, and use it as the primary seed liquid; the formula of the liquid PDA medium is as follows: Potato 200 grams, 20 grams of glucose, 1000 ml of tap water, natural pH,

[0023] (2) Take the seed solution obtained in step (1), inoculate it into the liquid PDA medium in a 20L fermenter with an inoculum volume of 5%, and culture it with aeration at 28°C until the exponential phase, as the secondary seed solution,

[0024] (3) Inoculate the secondary seed liquid into the YPDA medium of a 200L fermenter, cultivate to the stationary phase, and add 3-indoleacetic acid solution with a concentration of 0.8% by weight (3-indoleacetic acid is saturated with bicarbonate Dissolved in sodium solution or ...

Embodiment 3

[0027] The application of inoculating the bacterial strain with the function of synthesizing IBA into the fermented liquid of IAA-producing microorganisms to synthesize IBA.

[0028] (1) Microbial fermentation of IAA: Inoculate the IAA-producing strain Enterobacter LY6 (preservation number CGMCC NO 9539) into the medium (tryptone 0.8%, yeast extract 0.4%, NaCl 0.7%, glucose 2%, fructose 0.7%, L - 0.12% tryptophan, 0.02% sodium pyruvate, 0.01% copper sulfate, 1L water, pH 7.2), sterilized for 15 minutes after fermentation, and the sterilized fermentation broth was used for the synthesis of the yeast with the function of synthesizing IBA IBA.

[0029] (2) Preparation of yeast seed liquid: inoculate a ring of the yeast LY1 into 50 ml of liquid PDA medium from a slant preservation tube, place it in a shaker at 28°C, shake at 150 rpm until the exponential phase, and use it as the primary seed liquid; The primary seed solution was inoculated into the liquid PDA medium in a 20L ferm...

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PUM

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Abstract

The present invention is a yeast capable of synthesizing 3-indole butyric acid and its application in fermentative preparation of 3-indole butyric acid, which solves the problems of complex chemical synthesis process and environmental pollution of 3-indole butyric acid. Preparation of yeast with the function of synthesizing 3-indolebutyric acid, the Latin name is Saccharomyces sp., deposit unit: General Microbiology Center of China Committee for the Collection of Microbial Cultures, deposit date: April 11, 2017, deposit number: CGMCC NO .14011. The bacterial strain can convert the IAA prepared by microbial fermentation into IBA, and the fermented liquid is filtered, concentrated and crystallized to obtain the IBA.

Description

technical field [0001] The invention relates to a yeast and a method for preparing 3-indolebutyric acid by means of microbial fermentation. Background technique [0002] 3-Indolebutyric acid (IBA) and 3-indoleacetic acid (IAA), both are auxins naturally present in plants, can promote cell division and cell growth, induce the formation of adventitious roots, increase fruit setting, prevent fruit drop, and change The ratio of male and female flowers, etc. Widely used in agricultural and forestry production. There are many reports on the chemical synthesis of 3-indole butyric acid. At present, there are mainly two methods, one is obtained by condensation of indole and γ-butyrolactone at the reflux temperature of tetralin, and the other is obtained by indole It reacts with Grignard reagent and α-chloropropionitrile to produce 3-butyronitrile indole, which is then hydrolyzed by NaOH and reacted with hydrochloric acid. The chemical synthesis method has many steps, and the react...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12P17/10C12R1/85
CPCC12P17/10C12N1/185C12R2001/85
Inventor 王强锋侯勇曾显斌朱彭玲夏中梅王赟曾芸黄秀辉胡甦陈浩
Owner SICHUAN LANYUE SCI & TECH
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