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Bacillus subtilis hmb26553 and its application

A technology of Bacillus subtilis and strains, applied in the field of biological control, can solve the problems of affecting the growth and development of cotton, high drug resistance and cost, poor chemical control effect, etc., and achieves less drug resistance, good drug effect persistence, and good inhibitory effect Effect

Active Publication Date: 2020-02-11
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cotton blight seriously affects the growth and development of cotton, and often leads to the death of cotton seedlings after the onset, which is an urgent problem to be solved
[0003] Cotton blight is mainly controlled chemically, such as using chemical agents for seed dressing, coating or soaking before sowing, and spraying and root irrigation with chemical agents after sowing. Long-term medication is prone to problems such as drug resistance and high cost

Method used

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  • Bacillus subtilis hmb26553 and its application
  • Bacillus subtilis hmb26553 and its application
  • Bacillus subtilis hmb26553 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The preparation of embodiment 1HMB26553 microbial bacterial agent

[0050] Proceed as follows:

[0051] (1) Strain activation: Bacillus subtilis (Bacillus subtilis) strain HMB26553 (HMB26553) stored at -80°C was deposited in the General Microbiology Center of China Committee for Microbial Culture Collection on October 28, 2016, and the preservation number is CGMCC No.13211) is activated (30°C) on LB plate medium (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL), pick A single colony was cultured at 30°C for 12 hours on LB slant medium (its composition and weight ratio: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL) to obtain activated strains;

[0052] (2) Preparation of seed liquid: make LB liquid medium according to conventional methods (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, water 1000mL), and put it in a 250...

Embodiment 2

[0055] Embodiment 2 HMB26553 bacterial strain of the present invention is to the inhibitory action test of cotton solitaire mycelia growth

[0056] (1) R. solanacearum RH-2 tested: R. solanacearum RH-2 strain was collected from the diseased strain of cotton blight in Qiu County, Handan City, Hebei Province. It was isolated and purified by the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences. It was identified as Rhizoctonia solani by the Department of Plant Pathology, School of Plant Protection, University, and the pathogenicity test showed strong pathogenicity.

[0057] (2) Test method:

[0058] Plate assay test: first activate and cultivate Rhizoctonia solani RH-2 on a PDA plate for 3 days, and then use a puncher to Punch holes to make bacterial flakes, transfer the flakes of R. solani solanum to the center of another PDA plate, then spot the activated Bacillus subtilis HMB26553 at a place 2.0 cm away from the flakes of the indicator bacter...

Embodiment 3

[0063] Embodiment 3 The comparative test of the control effect of HMB26553 bacterial strain of the present invention to cotton blight

[0064] (1) Test treatment:

[0065] (1) Microbial agent: the HMB26553 liquid preparation prepared in Example 1 was diluted 50 times with water.

[0066] (2) Blank control: clear water

[0067] (2) Test method:

[0068] Mix vermiculite and cotton field soil at a ratio of 1:1, then sterilize at 121°C for 1 hour, and then sterilize again under the same conditions the next day. Inoculate Rhizoctonia solani RH-2 mycelia section suspension (about 10 in 500 gram soils) 7 per milliliter) 10 milliliters, stir evenly, fill in a plastic flower pot with a diameter of 20 cm, and compact it. The cotton variety Jifeng 106 was selected, and the seeds were soaked with the 50-fold water dilution of the HMB26553 liquid preparation prepared in Example 1 for half an hour before sowing; the seeds were soaked in clear water for half an hour as a blank control. ...

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Abstract

The invention discloses a Bacillus subtilis strain HMB26553, which is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and its preservation number is CGMCC No.13211. The invention also discloses the microbial inoculum produced by the bacterial strain and their application in preventing and treating plant diseases such as cotton blight. The Bacillus subtilis HMB26553 of the present invention has a high control effect on cotton blight, with an average control effect of more than 90.0%; secondly, the drug effect of the microbial agent is long-lasting, and there is no problem of environmental pollution; in addition, HMB26553 has a wide antibacterial spectrum , in addition to cotton blight, also has a good inhibitory effect on cotton Verticillium dahliad, cotton fusarium wilt, tomato Botrytis cinerea and cucumber target spot fungus.

Description

technical field [0001] The invention belongs to the field of biological control, and in particular relates to a bacillus subtilis HMB26553, microbial agents produced by the bacillus subtilis, and their application in preventing and treating cotton blight and other diseases. Background technique [0002] Cotton blight is a cotton seedling disease caused by Rhizoctonia solani Kühn. Its main symptoms are: cotton seeds are infected before germination and cause rotten seeds, and cotton seeds germinate before they emerge. Infected to cause rotten buds; Cotton seedlings are damaged after being unearthed, and yellow-brown lesions are formed near the base of the soil surface at the initial stage, and the lesions gradually expand and surround the entire base, showing obvious constriction, and the diseased seedlings wilt, fall and die; The cortex remains in the soil, and only the sharp rattail-shaped xylem remains; after the cotyledons are damaged, yellow-brown irregular-shaped lesions...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/125
CPCA01N63/00C12N1/20C12N1/205C12R2001/125
Inventor 马平李社增鹿秀云郭庆港张晓云王莹王培培赵卫松
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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