Method for testing greenhouse gas emission of indoor culture soil

Abstract

The invention relates to a method for testing greenhouse gas emission of indoor culture soil. The method is implemented by using a gas exchange and culture gas production integral device; the gas exchange culture gas production integral device comprises a gas exchange device and a culture gas production device which is connected with the gas exchange device; the testing method comprises steps of opened culture of soil, gas exchange, sealed culture and gas collection. By adopting the method, the influence of gases generated from the soil at an early stage upon sealed culture gas emission is eliminated, sealed culture at normal pressure is achieved, meanwhile the problem that a target gas is absorbed by negative pressure in sampling is overcome, in addition, culture and gas collection of multiple culture flasks can be completed at one time, the initiation culture concentration is accordant, and the working efficiency is high.

Description

technical field [0001] The invention relates to a method for measuring greenhouse gas emissions from indoor cultured soil, and belongs to the technical field of greenhouse gas monitoring research. Background technique [0002] The main driver of global warming is the increase in the concentration of greenhouse gases in the atmosphere, carbon dioxide (CO 2 ), methane (CH 4 ) and nitrous oxide (N 2 O) is the three most important greenhouse gases in the atmosphere. Their contribution to global warming accounts for nearly 80%, and their atmospheric concentrations increase at an average annual rate of 0.5%, 0.8% and 0.3%, respectively. Among all greenhouse gas emission sources, soil is the most important emission source, and it is estimated that 5%-20% of CO is in the atmosphere every year 2 , 15%-30% CH 4 and 80%-90% of N 2 O originates from the soil. Therefore, scholars at home and abroad have carried out relevant observations and research on the three main greenhouse gas...

AI Technical Summary

Problems solved by technology

In order to facilitate batch indoor cultivation, smaller glass bottles are often used as culture bottles in experiments, such as 250ml triangular flasks or 100ml sterile serum bottles. If the above method is followed, the problem of interfering with the cultivation of soil gas emissions may occur. affect the accuracy of the experimental results

The main reasons for the impact are as follows: 1) Due to the small opening of the culture bottle, the gas released by the soil culture should not be released, resulting in an increase in the gas concentration and affecting the gas discharge during the culture

2) During the closed cultivation process, the gas released by the soil will cause the gas pressure in the cultivation device to be higher than the atmospheric pressure, and the natural concentration gradient at the interface between the soil and the bottom atmosphere cannot be maintained, and the obtained results are difficult to describe the actual gas release of the soil

3) Use glass bottles for cultivation. If the sampling volume is too large, it will cause negative pressure in the culture bottle and the soil sample will absorb gas.

4) Collect gas samples for analysis before and after airtightness, the workload is heavy and the analysis cost is high

5) In the experiment, a thin needle is often inserted into the silicone plug to get air, which may easily cause needle blockage and long-term use of the silicone plug to leak air.

[0004] At present, there is no publication of relevant literature on a more realistic, effective, and closer to actual method for measuring greenhouse gas emissions from indoor cultivation soils.

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